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The trithorax gene, a trans-acting regulator of the bithorax complex in Drosophila, encodes a protein with zinc-binding domains

Mazo, Alexander M. and Huang, Der-Hwa and Mozer, Brian A. and Dawid, Igor B. (1990) The trithorax gene, a trans-acting regulator of the bithorax complex in Drosophila, encodes a protein with zinc-binding domains. Proceedings of the National Academy of Sciences of the United States of America, 87 (6). pp. 2112-2116. ISSN 0027-8424. https://resolver.caltech.edu/CaltechAUTHORS:20141212-132435522

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Abstract

The trithorax (trx) gene functions in segment determination in Drosophila through interaction with genes of the bithorax complex and Antennapedia complex. Genetic evidence suggests that trx may be considered a positive regulator of homeotic genes. Sequencing of cDNAs corresponding to the entire trx transcription unit revealed the existence of an unusually long open reading frame encoding 3759 amino acids. The main features of the predicted trx protein are several cysteine-rich regions which can be folded into zinc finger-like domains. Cysteine-rich portions expressed from trx cDNAs in Escherichia coli are capable of zinc binding in vitro, suggesting a possible function for the trx product as a metal-dependent DNA-binding protein. Analysis of trx mutant embryos with antibody to the Ultrabithorax (Ubx) gene product showed decreased staining in parasegment 6 of the ventral nerve cord of late embryos. However, expression of Ubx was not affected in embryos carrying the lethal mutation trxE3, in which one of the putative zinc finger-like domains of the trx protein is deleted. This differential effect of the E3 mutation suggests that trx exhibits other function(s) besides those involved in the regulation of Ubx expression in the ventral nerve cord of the embryo.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1073/pnas.87.6.2112 DOIArticle
http://www.pnas.org/content/87/6/2112PublisherArticle
Additional Information:© 1990 National Academy of Sciences. Contributed by Igor B. Dawid, December 29, 1989. We thank S. Haynes and J. Kennison for advice and help throughout; S. Haynes, J. Kennison, and J. Kassis for comments on the manuscript; G. Michaels for help with computer searches; N. Patel and C. Goodman for supplying the anti-Ubx hybridoma line of White and Wilcox (20); M. Barbarosa for supplying partially purified TrpE-E6 fusion protein and helpful discussions; and P. Beachy, S. Carroll, T. Karr, and J. Kassis for providing staining protocols. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:homeotic gene; Ultrabithorax gene; zinc finger
Issue or Number:6
Record Number:CaltechAUTHORS:20141212-132435522
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20141212-132435522
Official Citation:A M Mazo, D H Huang, B A Mozer, and I B Dawid The trithorax gene, a trans-acting regulator of the bithorax complex in Drosophila, encodes a protein with zinc-binding domains PNAS 1990 87 (6) 2112-2116
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:52769
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:12 Dec 2014 22:26
Last Modified:03 Oct 2019 07:44

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