Einsle, Oliver and Andrade, Susana L. A. and Dobbek, Holger and Meyer, Jacques and Rees, Douglas C. (2007) Assignment of Individual Metal Redox States in a Metalloprotein by Crystallographic Refinement at Multiple X-ray Wavelengths. Journal of the American Chemical Society, 129 (8). pp. 2210-2211. ISSN 0002-7863. PMCID PMC2527600. doi:10.1021/ja067562o. https://resolver.caltech.edu/CaltechAUTHORS:20141212-144040144
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Abstract
A method is presented to derive anomalous scattering contributions for individual atoms within a protein crystal by collecting several sets of diffraction data at energies spread along an X-ray absorption edge of the element in question. The method has been applied to a [2Fe:2S] ferredoxin model system with localized charges in the reduced state of the iron−sulfur cluster. The analysis shows that upon reduction the electron resides at the iron atom closer to the protein surface. The technique should be sufficiently sensitive for more complex clusters with noninteger redox states and is generally applicable given that crystals are available.
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Contact Email Address: | phoebe@caltech.edu | |||||||||||||||
Additional Information: | © 2007 American Chemical Society Received October 23, 2006 Diffraction data were collected at SSRL, Stanford, and EMBL/DESY, Hamburg, Germany. This work was supported in part by EMBO YIP (O.E.), DFG (O.E., S.L.A.A.), CNRS and UJF (J.M.), and by NIH Grant GM45162 (D.C.R.). | |||||||||||||||
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Issue or Number: | 8 | |||||||||||||||
PubMed Central ID: | PMC2527600 | |||||||||||||||
DOI: | 10.1021/ja067562o | |||||||||||||||
Record Number: | CaltechAUTHORS:20141212-144040144 | |||||||||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20141212-144040144 | |||||||||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||||||||
ID Code: | 52778 | |||||||||||||||
Collection: | CaltechAUTHORS | |||||||||||||||
Deposited By: | SWORD User | |||||||||||||||
Deposited On: | 22 Dec 2014 21:16 | |||||||||||||||
Last Modified: | 10 Nov 2021 19:43 |
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