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Thermal stability comparison of purified empty and peptide-filled forms of a class I MHC molecule

Fahnestock, Margaret L. and Tamir, Ilana and Narhi, Linda and Bjorkman, Pamela J. (1992) Thermal stability comparison of purified empty and peptide-filled forms of a class I MHC molecule. Science, 258 (5088). pp. 1658-1662. ISSN 0036-8075. https://resolver.caltech.edu/CaltechAUTHORS:20141215-124418088

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Abstract

A secreted form of a class I major histocompatibility complex (MHC) molecule was denatured and renatured in vitro in the absence of peptide. The resulting empty class I heterodimer was immunologically reactive and structurally similar to a heterodimer renatured in the presence of an appropriate restricted peptide. Thermal stability profiles indicated that the two forms of heterodimer differed in their resistance to denaturation by heat but that a significant portion of the empty class I heterodimers had a native conformation at physiological temperatures. Free energies calculated from these data gave a direct measure of the stabilization of the class I MHC molecule that resulted from peptide binding.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1126/science.1360705DOIArticle
http://www.sciencemag.org/content/258/5088/1658.abstractPublisherArticle
http://www.jstor.org/stable/2882076JSTORArticle
ORCID:
AuthorORCID
Bjorkman, Pamela J.0000-0002-2277-3990
Additional Information:© 1992 American Association for the Advancement of Science. Received 1 July 1992, accepted 2 October 1992. We thank M. Raghavan for doing the acid-elution experiments, D. C. Rees and T. Arakawa for helpful discussions, C. Bebbington for the glutamine synthetase amplification vector; J. Moore for help with CD analysis, D. Penny tor assistance with tissue culture, M. Blum and R. Strong for help with graphics, and D. C. Rees, N. Davidson, and M. Raghavan for critical reading of the manuscript Supported by NIH (Al28931 to P.J.B.), and an NIH postdoctoral fellowship (M.L.F.). P.J.B is a scholar of the Pew Charitable Trusts and the Cancer Research Institute.
Funders:
Funding AgencyGrant Number
NIHAl28931
NIH Postdoctoral FellowshipUNSPECIFIED
Issue or Number:5088
Record Number:CaltechAUTHORS:20141215-124418088
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20141215-124418088
Official Citation:Thermal stability comparison of purified empty and peptide-filled forms of a class I MHC molecule ML Fahnestock, I Tamir, L Narhi, and PJ Bjorkman Science 4 December 1992: 258 (5088), 1658-1662. [DOI:10.1126/science.1360705]
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:52817
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:15 Dec 2014 23:23
Last Modified:03 Oct 2019 07:44

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