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NICER elements: a family of nerve growth factor-inducible cAMP-extinguishable retrovirus-like elements

Cho, Kyung-Ok and Minsk, Brian and Wagner, John A. (1990) NICER elements: a family of nerve growth factor-inducible cAMP-extinguishable retrovirus-like elements. Proceedings of the National Academy of Sciences of the United States of America, 87 (10). pp. 3778-3782. ISSN 0027-8424. PMCID PMC53986. doi:10.1073/pnas.87.10.3778.

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We have shown previously that the transcription of the gene designated d5 is induced by nerve growth factor (NGF) in rat adrenal pheochromocytoma PC-12 cells and that this NGF induction is repressed by cAMP. In this paper we demonstrate that d5 is a member of a gene family that contains several hundred members, which is closely related to retroviruses and retrotransposons, as demonstrated by the following observations: (i) the original d5 cDNA hybridized to numerous restriction fragments in genomic DNA; (ii) d5 cDNA hybridized to genomic clones with various intensities, and genomic clones can be isolated with a frequency suggesting that this family includes several hundred members; and (iii) there were minor sequence variations in four independently isolated cDNA clones that were homologous to d5 cDNA. Primer extension studies show that initiation of the 5.7-kilobase d5 mRNA(s) occurs at a unique site relative to a synthetic primer. The 5' end of the cDNA sequence was homologous to Rasheed rat sarcoma virus; and a genomic clone contained several elements that are typical of a long terminal repeat (LTR), including a CCAAT box, a TATA box, a primer binding site, a poly(A) addition signal, and a poly(A) addition site. Furthermore, there is a LTR at the 3' end of at least one of the genes in this family, and there appeared to be a four-base duplication at the probable site of integration into host DNA. Since several members of this family retain responses to NGF and cAMP, we conclude that the regulatory elements present in the LTR have been conserved in many members of this family. We have named this family of genes the NICER elements because they are a family of NGF-inducible cAMP-extinguishable retrovirus-like elements.

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Additional Information:© 1990 National Academy of Sciences. Communicated by Arthur B. Pardee, January 25, 1990 (received for review October 28, 1989). We thank Robert Hawley for his initial involvement in isolating and characterizing cDNA clones and Paul Kaplan and Prescott Deininger for helpful advice and criticism. These studies were supported by a grant from the National Institutes of Health (CA22427 and 2S07RR05526-27). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
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Subject Keywords:PC-12 cells; long terminal repeat; Rasheed rat sarcoma virus; retrotransposon
Issue or Number:10
PubMed Central ID:PMC53986
Record Number:CaltechAUTHORS:20141215-131127851
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Official Citation:K O Cho, B Minsk, and J A Wagner NICER elements: a family of nerve growth factor-inducible cAMP-extinguishable retrovirus-like elements. PNAS 1990 87 (10) 3778-3782; doi:10.1073/pnas.87.10.3778
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:52819
Deposited By: Tony Diaz
Deposited On:15 Dec 2014 23:22
Last Modified:10 Nov 2021 19:44

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