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Regulation of the yeast DNA replication genes through the Mlu I cell cycle box is dependent on SWI6

Verma, Rati and Smiley, Jean and Andrews, Brenda and Campbell, Judith L. (1992) Regulation of the yeast DNA replication genes through the Mlu I cell cycle box is dependent on SWI6. Proceedings of the National Academy of Sciences of the United States of America, 89 (20). pp. 9479-9483. ISSN 0027-8424. https://resolver.caltech.edu/CaltechAUTHORS:20141215-133154866

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Abstract

In Saccharomyces cerevisiae, at least 17 DNA replication genes are coordinately expressed at the G_1/S boundary during the cell cycle. All of these genes have the DNA sequence element ACGCGT in their 5' upstream regulatory regions. This sequence has been shown to be essential for periodic expression of the POL1, CDC9, and TMP1 genes. The cyclin (CLN1 and CLN2) and HO genes are another subset of genes that are expressed with the same timing as the DNA replication genes. Their periodic expression requires the participation of two well-characterized transcriptional activators: the SWI4 and SWI6 gene products. In this study, we present evidence that SWI6 contributes to the regulation of DNA replication genes as well. Surprisingly, a preferential requirement for SWI6 over SWI4 is observed in our studies of ACGCGT-dependent reporter gene expression in vivo. This selectivity has not been observed for the other G_1/S genes. Correlating with the in vivo results, protein-DNA complexes formed in vitro on multimeric ACGCGT elements are either abolished or reduced in swi6Δ deletion mutants.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1073/pnas.89.20.9479 DOIArticle
http://www.pnas.org/content/89/20/9479PublisherArticle
Additional Information:© 1992 National Academy of Sciences. Communicated by John Abelson, June 30, 1992. This work was supported by United States Public Health Service Grant GM25508 and the National Cancer Institute of Canada. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
United States Public Health ServiceGM25508
National Cancer Institute of CanadaUNSPECIFIED
Issue or Number:20
Record Number:CaltechAUTHORS:20141215-133154866
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20141215-133154866
Official Citation:R Verma, J Smiley, B Andrews, and J L Campbell Regulation of the yeast DNA replication genes through the Mlu I cell cycle box is dependent on SWI6. PNAS 1992 89 (20) 9479-9483; doi:10.1073/pnas.89.20.9479
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:52820
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:15 Dec 2014 23:21
Last Modified:03 Oct 2019 07:44

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