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Myt1: A Membrane-Associated Inhibitory Kinase That Phosphorylates Cdc2 on Both Threonine-14 and Tyrosine-15

Mueller, Paul R. and Coleman, Thomas R. and Kumagai, Akiko and Dunphy, William G. (1995) Myt1: A Membrane-Associated Inhibitory Kinase That Phosphorylates Cdc2 on Both Threonine-14 and Tyrosine-15. Science, 270 (5233). pp. 86-90. ISSN 0036-8075. doi:10.1126/science.270.5233.86.

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Cdc2 is the cyclin-dependent kinase that controls entry of cells into mitosis. Phosphorylation of Cdc2 on threonine-14 and tyrosine-15 inhibits the activity of the enzyme and prevents premature initiation of mitosis. Although Wee1 has been identified as the kinase that phosphorylates tyrosine-15 in various organisms, the threonine-14-specific kinase has not been isolated. A complementary DNA was cloned from Xenopus that encodes Myt1, a member of the Wee1 family that was discovered to phosphorylate Cdc2 efficiently on both threonine-14 and tyrosine-15. Myt1 is a membrane-associated protein that contains a putative transmembrane segment. Immunodepletion studies suggested that Myt1 is the predominant threonine-14-specific kinase in Xenopus egg extracts. Myt1 activity is highly regulated during the cell cycle, suggesting that this relative of Wee1 plays a role in mitotic control.

Item Type:Article
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URLURL TypeDescription DOIArticle
Kumagai, Akiko0000-0003-2422-8053
Dunphy, William G.0000-0001-7598-8939
Additional Information:© 1995 American Association for the Advancement of Science. Received 29 June 1995; accepted 6 September 1995. We are grateful to D. Morgan (University of California, San Francisco) for supplying the baculovirus transfer vector, M. Solomon (Yale University) for providing Cdc2 mutant plasmids. J. Kuang (M. D. Anderson Cancer Center) for supplying MPM-2 antibodies, and B. Wold and members of the W.G.D. group for critical reading of this manuscript . Supported by grants from NIH, Lucille P. Markey Charitable Trust, and NSF. P.R.M. and T.R.C. were supported by fellowships from the Helen Hay Whitney Foundat ion and NIH (National Research Service Awards), respectively. P.R.M. is an associate and W.G.D. is an investigator at the Howard Hughes Medical Institute.
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Lucille P. Markey Charitable TrustUNSPECIFIED
Helen Hay Whitney FoundationUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Issue or Number:5233
Record Number:CaltechAUTHORS:20141216-093746536
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Official Citation:Myt1: A Membrane-Associated Inhibitory Kinase That Phosphorylates Cdc2 on Both Threonine-14 and Tyrosine-15 Paul R. Mueller, Thomas R. Coleman, Akiko Kumagai, and William G. Dunphy Science 6 October 1995: 270 (5233), 86-90. [DOI:10.1126/science.270.5233.86]
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:52849
Deposited By: Tony Diaz
Deposited On:16 Dec 2014 17:47
Last Modified:10 Nov 2021 19:44

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