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dOct2, a Drosophila Oct transcription factor that functions in yeast

Prakash, Kulkarni and Fang, Xiang-Dong and Engelber, David and Behal, Amita and Parker, Carl S. (1992) dOct2, a Drosophila Oct transcription factor that functions in yeast. Proceedings of the National Academy of Sciences of the United States of America, 89 (15). pp. 7080-7084. ISSN 0027-8424. PMCID PMC49649. doi:10.1073/pnas.89.15.7080.

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Oct factors are members of the POU family of transcription factors that are shown to play important roles during development in mammals. Here we report the cDNA cloning and expression of a Drosophila Oct transcription factor. Whole mount in situ hybridization experiments revealed that the spatial expression patterns of this gene during embryonic development have not yet been observed for any other gene. In early embryogenesis, its transcripts are transiently expressed as a wide uniform band from 20% to 40% of the egg length, very similar to that of gap genes. This pattern progressively resolves into a series of narrower stripes followed by expression in 14 stripes. Subsequently, transcripts from this gene are expressed in the central nervous system and the brain. When expressed in the yeast Saccharomyces cerevisiae, this Drosophila factor functions as a strong, octamer-dependent activator of transcription. Our data strongly suggest possible functions for the Oct factor in pattern formation in Drosophila that might transcend the boundaries of genetically defined segmentation genes.

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Additional Information:© 1992 National Academy of Sciences. Communicated by Eric H. Davidson, April 14, 1992 (received for review February 25, 1992). We are grateful to Dr. Tao Hsieh for the embryonic Agt11 cDNA library, to Dr. Mike Muhich for the Drosophila TFIID clone, and to Drs. Judith Lengyel and Walter Gehring for their suggestions and protocols regarding the whole-mount in situ hybridizations. We thank Drs. Dali Ding and Susan Parkhurst for their help with the polytene chromosome hybridization experiments, Dr. Ebrahim Zandi for unpublished data, Dr. Steve Poole for sharing cDNA clones, and Dr. Steven Triezenberg for the VP16 plasmid. D.E. is supported by a Rothschild Fellowship (Israel). This work was supported by Grant GM42671 from the National Institutes of Health. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funding AgencyGrant Number
Rothschild Fellowship (Israel)UNSPECIFIED
Subject Keywords:Oct factor; POU domain; transactivator; embryonic development
Issue or Number:15
PubMed Central ID:PMC49649
Record Number:CaltechAUTHORS:20141216-143048530
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Official Citation:dOct2, a Drosophila Oct transcription factor that functions in yeast K Prakash, X D Fang, D Engelberg, A Behal, and C S Parker PNAS 1992 89 (15) 7080-7084
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:52893
Deposited By: Ruth Sustaita
Deposited On:16 Dec 2014 22:51
Last Modified:10 Nov 2021 19:45

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