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Identification and purification of a factor that binds to the Mlu I cell cycle box of yeast DNA replication genes

Verma, Rati and Patapoutian, Ardem and Gordon, Colin B. and Campbell, Judith L. (1991) Identification and purification of a factor that binds to the Mlu I cell cycle box of yeast DNA replication genes. Proceedings of the National Academy of Sciences of the United States of America, 88 (16). pp. 7155-7159. ISSN 0027-8424. http://resolver.caltech.edu/CaltechAUTHORS:20141216-151345665

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Abstract

In Saccharomyces cerevisiae, the genes encoding at least 10 enzymes involved in DNA replication are periodically expressed in the late G1 and S phases of the cell cycle. All of these genes have one copy or more of the sequence ACGCGT, which conforms to the recognition site for the Mlu I restriction endonuclease. For the CDC21, CDC9, and POL1 genes, the Mlu I site has been shown to be absolutely required for periodic transcription. Using nuclear extracts fractionated by conventional and oligonucleotide affinity chromatography, we have purified a 17-kDa protein that recognizes the Mlu I motif. Synthetic oligonucleotides containing mutated Mlu I sites do not bind the protein. In contrast, synthetic oligonucleotides derived from the CDC2, CDC6, and CDC21 genes, which are expressed with the same timing as POL1, bind purified protein efficiently.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1073/pnas.88.16.7155DOIArticle
http://www.pnas.org/content/88/16/7155.abstractPublisherArticle
Additional Information:© 1991 National Academy of Sciences. Communicated by John D. Roberts, May 15, 1991. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Subject Keywords:Saccharomyces cerevisiae; G1, phase; S phase; periodic transcription; DNA polymerase α
Record Number:CaltechAUTHORS:20141216-151345665
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20141216-151345665
Official Citation:Identification and purification of a factor that binds to the Mlu I cell cycle box of yeast DNA replication genes. R Verma, A Patapoutian, C B Gordon, and J L Campbell PNAS 1991 88 (16) 7155-7159; doi:10.1073/pnas.88.16.7155
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:52901
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:16 Dec 2014 23:26
Last Modified:16 Dec 2014 23:26

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