Yamamori, Tetsuo (1991) Localization of cholinergic differentiation factor/leukemia inhibitory factor mRNA in the rat brain and peripheral tissues. Proceedings of the National Academy of Sciences of the United States of America, 88 (16). pp. 7298-7302. ISSN 0027-8424. PMCID PMC52282. doi:10.1073/pnas.88.16.7298. https://resolver.caltech.edu/CaltechAUTHORS:20141216-152140284
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Abstract
Sympathetic neurons display considerable plasticity in the neurotransmitter and neuropeptide phenotypes they express in vitro and in vivo. The cholinergic differentiation factor (CDF, also known as leukemia inhibitory factor, LIF) induces cultured rat sympathetic neurons to become cholinergic, without affecting their survival or growth. To understand the role of this factor in normal development, it is essential to determine where it is produced in situ. To localize CDF/LIF mRNA, a semiquantitative, reverse transcription-polymerase chain reaction method was employed. Actin and tubulin mRNA were used as internal controls, and two different sets of CDF/LIF primers were compared. In postnatal rat peripheral tissues, CDF/LIF mRNA was selectively localized in the target area of developing, sympathetic cholinergic neurons; the mRNA was not detected in the targets of sympathetic noradrenergic neurons. This finding supports the hypothesis that CDF/LIF is a target-derived neuronal differentiation factor. In postnatal rat brain, CDF/LIF mRNA is localized selectively in two parts of the visual system, visual cortex and superior colliculus. Thus, CDF/LIF may play a role in this system as well.
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| Additional Information: | © 1991 National Academy of Sciences. Communicated by Masakazu Konishi, May 30, 1991. I thank Colin McDonald for essential help in brain dissection and for comments on the manuscript; Story Landis, Mahendra Rao, Ming-Ji Fann, Norman Davidson, and Kai Zinn for useful comments and suggestions; Robert Schotzinger for help with dissection; and David Anderson for the generous use of his equipment and for comments on the manuscript. I thank Paul Patterson, in whose laboratory this work was carried out, for encouragement and advice and for help with the manuscript. This research was supported by grants from the National Institute of Neurological Disorders and Stroke (Javits Neuroscience Investigator Award) and the Alzheimer's Disease and Related Disorders Association to Paul H. Patterson. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact. | |||||||||
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| Subject Keywords: | sympathetic neuron; sweat gland; phenotypic choice; cytokine | |||||||||
| Issue or Number: | 16 | |||||||||
| PubMed Central ID: | PMC52282 | |||||||||
| DOI: | 10.1073/pnas.88.16.7298 | |||||||||
| Record Number: | CaltechAUTHORS:20141216-152140284 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20141216-152140284 | |||||||||
| Official Citation: | Localization of cholinergic differentiation factor/leukemia inhibitory factor mRNA in the rat brain and peripheral tissues. T Yamamori PNAS 1991 88 (16) 7298-7302; doi:10.1073/pnas.88.16.7298 | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 52905 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Ruth Sustaita | |||||||||
| Deposited On: | 16 Dec 2014 23:32 | |||||||||
| Last Modified: | 10 Nov 2021 19:45 |
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