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The CDC7 protein of Saccharomyces cerevisiae is a phosphoprotein that contains protein kinase activity

Yoon, Hye-Joo and Campbell, Judith L. (1991) The CDC7 protein of Saccharomyces cerevisiae is a phosphoprotein that contains protein kinase activity. Proceedings of the National Academy of Sciences of the United States of America, 88 (9). pp. 3574-3578. ISSN 0027-8424. https://resolver.caltech.edu/CaltechAUTHORS:20141216-152858252

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Abstract

The CDC7 protein of Saccharomyces cerevisiae may be involved in the G_1/S-phase transition and/or in the initiation of mitotic DNA synthesis. The CDC7 gene has two in-frame AUG codons as possible translation start sites, which would produce 58- and 56-kDa proteins, respectively. Both p58 and p56 derived from recombinant plasmids complement the temperature-sensitive growth defect of the cdc7-1 allele. To determine the biochemical function of the CDC7 protein, the CDC7 gene was cloned and polyclonal antibodies were produced against the CDC7 protein. CDC7 immune complexes prepared from yeast with these antibodies phosphorylate histone H1. Kinase activity is thermolabile in strains carrying the cdc7-1 temperature-sensitive mutant allele and is elevated >10-fold in strains carrying plasmids overexpressing either p56 or p58, confirming that the kinase in the immunoprecipitates is the CDC7 gene product. In addition, we show that CDC7 is a phosphoprotein itself. Indirect immunofluorescence and biochemical fractionation show that the CDC7 protein is present at relatively high concentrations in the nucleus compared with the cytoplasm, suggesting that nuclear proteins may be substrates for the CDC7 protein.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1073/pnas.88.9.3574DOIArticle
http://www.pnas.org/content/88/9/3574.abstractPublisherArticle
Additional Information:© 1991 National Academy of Sciences. Communicated by Charles C. Richardson, January 28, 1991 (received for review November 27, 1990). We appreciate the help of Michael W. Clark with the indirect immunofluorescence microscopy. We thank Ambrose Jong for preparing the cdc7-1 ura3-52 strain used to isolate the CDC7 gene. This work was supported in part by a Developmental Biology Grant from the Lucille P. Markey Charitable Trust and the National Science Foundation Grant CHE-8509637. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
Lucille P. Markey Charitable Trust Developmental Biology GrantUNSPECIFIED
NSFCHE-8509637
Subject Keywords:cell division cycle; S phase; phosphorylation; DNA replication
Issue or Number:9
Record Number:CaltechAUTHORS:20141216-152858252
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20141216-152858252
Official Citation:The CDC7 protein of Saccharomyces cerevisiae is a phosphoprotein that contains protein kinase activity. H J Yoon and J L Campbell PNAS 1991 88 (9) 3574-3578; doi:10.1073/pnas.88.9.3574
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:52907
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:16 Dec 2014 23:42
Last Modified:03 Oct 2019 07:45

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