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Identification of Dictyostelium G_ɑ genes expressed during multicellular development

Hadwiger, Jeffrey and Wilkie, Thomas M. and Strathmann, Michael and Firtel, Richard A. (1991) Identification of Dictyostelium G_ɑ genes expressed during multicellular development. Proceedings of the National Academy of Sciences of the United States of America, 88 (18). pp. 8213-8217. ISSN 0027-8424. PMCID PMC52477. doi:10.1073/pnas.88.18.8213.

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Guanine nucleotide-binding protein (G protein)-mediated signal transduction constitutes a common mechanism by which cells receive and respond to a diverse set of environmental signals. Many of the signals involved in the developmental life cycle of the slime mold Dictyostelium have been postulated to be transduced by such pathways and, in some cases, these pathways have been demonstrated to be dependent on specific G proteins. Using the polymerase chain reaction, we have identified two additional Dictyostelium G_ɑ genes, G_ɑ4 and G_ɑ5, that are developmentally regulated. Transcripts from both of these genes are primarily expressed during the multicellular stages of development, suggesting possible roles in cell differentiation or morphogenesis. The entire G_ɑ 4 gene was sequenced and found to encode a protein consisting of 345 amino acids. The G_ɑ4 subunit is homologous to other previously identified G_ɑ subunits, including the Dictyostelium Gɑ1 (43% identity) and G_ɑ2 (41% identity) subunits. However, the G_ɑ4 subunit contains some unusual sequence divergences in residues highly conserved among most eukaryotic G_ɑ subunits, suggesting that G_ɑ4 may be a member of another class of G_ɑ subunits.

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Alternate Title:Identification of Dictyostelium Gɑ genes expressed during multicellular development
Additional Information:© 1991 National Academy of Sciences. Communicated by Melvin I. Simon, May 15, 1991. We thank J. Dynes for advice on library construction, P. Howard for advice on PCR and KAx-3 cDNA, J. Powell for some of the oligonucleotides, and A. Cubitt for helpful discussions. J.A.H. is supported by an American Cancer Society postdoctoral fellowship (PF3285) and T.M.W. is supported by a National Institutes of Health postdoctoral fellowship (GM11576). This work was supported by U.S. Public Health Service grants to R.A.F. (GM30693 and GM37830) and to M. I. Simon (GM34236).
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American Cancer SocietyPF3285
Issue or Number:18
PubMed Central ID:PMC52477
Record Number:CaltechAUTHORS:20141217-080557398
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Official Citation:Identification of Dictyostelium G alpha genes expressed during multicellular development J A Hadwiger, T M Wilkie, M Strathmann, and R A Firtel PNAS 1991 88 (18) 8213-8217
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:52933
Deposited By: Ruth Sustaita
Deposited On:17 Dec 2014 17:44
Last Modified:10 Nov 2021 19:45

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