A Caltech Library Service

Membrane depolarization induces p140^(trk) and NGF responsiveness, but not p75^(LNGFR), in MAH cells

Birren, Susan J. and Verdi, Joseph M. and Anderson, David J. (1992) Membrane depolarization induces p140^(trk) and NGF responsiveness, but not p75^(LNGFR), in MAH cells. Science, 257 (5068). pp. 395-397. ISSN 0036-8075. doi:10.1126/science.1321502.

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item:


Nerve growth factor (NGF) is required for the maturation and survival of sympathetic neurons, but the mechanisms controlling expression of the NGF receptor in developing neuroblasts have not been defined. MAH cells, an immortalized sympathoadrenal progenitor cell line, did not respond to NGF and expressed neither low-affinity NGF receptor (p75) nor p140trk messenger RNAs. Depolarizing concentrations of potassium chloride, but none of a variety of growth factors, induced expression of p140trk but not p75 messenger RNA. A functional response to NGF was acquired by MAH cells under these conditions, suggesting that expression of p75 is not essential for this response. Depolarization also permitted a relatively high proportion of MAH cells to develop and survive as neurons in fibroblast growth factor and NGF. These data establish a relation between electrical activity and neurotrophic factor responsiveness in developing neurons, which may operate in the functioning of the mature nervous system as well.

Item Type:Article
Related URLs:
URLURL TypeDescription DOIArticle
Anderson, David J.0000-0001-6175-3872
Additional Information:© 1992 American Association for the Advancement of Science. 6 April 1992; Accepted 5 June 1992. We thank L. Parada for the Trk cDNA probe, E. M. Johnson for monoclonal antibody 192-1g, M. Chao for the p75 probe, and G. Yancopoulos (Regeneron) for BDNF and NT-3; R. Diamond for performing flow cytometric analysis; S. Padilla and M. Sylber for technical assistance; D. Stemple for helpful comments and suggestions; and K. Zinn and P. Patterson for comments on the manuscript. This work was supported by NIH grant NS23476. S.J.B. was supported by funds from the PEW Neurosciences program. D.J.A. is an assistant investigator and J.V. is an associate of the Howard Hughes Medical Institute.
Funding AgencyGrant Number
Pew Charitable TrustUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Issue or Number:5068
Record Number:CaltechAUTHORS:20141222-121310383
Persistent URL:
Official Citation:Birren, S., Verdi, J., & Anderson, D. (1992). Membrane depolarization induces p140trk and NGF responsiveness, but not p75LNGFR, in MAH cells. Science, 257(5068), 395-397. doi: 10.1126/science.1321502
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53094
Deposited On:22 Dec 2014 20:52
Last Modified:10 Nov 2021 19:47

Repository Staff Only: item control page