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Domain of a yeast site-specific recombinase (Flp) that recognizes its target site

Chen, Jing-Wen and Evans, Barbara R. and Yang, Sang-Hwa and Teplow, David B. and Jayaram, Makkuni (1991) Domain of a yeast site-specific recombinase (Flp) that recognizes its target site. Proceedings of the National Academy of Sciences of the United States of America, 88 (14). pp. 5944-5948. ISSN 0027-8424. https://resolver.caltech.edu/CaltechAUTHORS:20141222-131437336

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Abstract

Binding of a partial proteolytic digest by V8 enzyme of the yeast site-specific recombinase Flp to its target site gives rise to DNA-protein complexes that are smaller than those produced by the full-sized protein. The smallest of these complexes (occupancy of one peptide monomer per site) contains either one of two polypeptides (32 and 28 kDa) of the V8 digestion mixture. The amino termini of both polypeptides map to Ser-129 of Flp, corresponding to V8 cleavage at Glu-128. The relative mobilities of the complexes formed by the V8 peptides indicate that they lack the sharp substrate bend that is characteristic of Flp-derived complexes. A hybrid protein consisting of the amino-terminal one-third of the R recombinase (from Zygosaccharomyces rouxii) and the carboxyl-terminal two-thirds of Flp recognizes the Flp target site.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1073/pnas.88.14.5944DOIArticle
http://www.pnas.org/content/88/14/5944PublisherArticle
Additional Information:© 2014 National Academy of Sciences. Communicated by Esmond E. Snell, April 1, 1991. We acknowledge the gift of the R recombinase expression plasmid from H. Araki and Y. Oshima. We thank Lei Zheng, Christine Acklin, and Tammy Bauer for excellent technical assistance. This work was supported by National Institutes of Health funds to M.J. and by National Science Foundation Grant DIR 86-18937 to D.B.T.
Funders:
Funding AgencyGrant Number
NIHUNSPECIFIED
NSFDIR 86-18937
Issue or Number:14
Record Number:CaltechAUTHORS:20141222-131437336
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20141222-131437336
Official Citation:Chen, J. W., Evans, B. R., Yang, S. H., Teplow, D. B., & Jayaram, M. (1991). Domain of a yeast site-specific recombinase (Flp) that recognizes its target site. Proceedings of the National Academy of Sciences, 88(14), 5944-5948.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53101
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:22 Dec 2014 22:08
Last Modified:03 Oct 2019 07:47

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