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Cloning and expression of a rat brain GABA transporter

Guastella, John and Nelson, Nathan and Nelson, Hannah and Czyzyk, Linda and Keynan, Shoshi and Miedel, May C. and Davidson, Norman and Lester, Henry A. and Kanner, Baruch (1990) Cloning and expression of a rat brain GABA transporter. Science, 249 (4974). pp. 1303-1306. ISSN 0036-8075. doi:10.1126/science.1975955.

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A complementary DNA clone (designated GAT-1) encoding a transporter for the neurotransmitter gamma-aminobutyric acid (GABA) has been isolated from rat brain, and its functional properties have been examined in Xenopus oocytes. Oocytes injected with GAT-1 synthetic messenger RNA accumulated [^3H]GABA to levels above control values. The transporter encoded by GAT-1 has a high affinity for GABA, is sodium-and chloride-dependent, and is pharmacologically similar to neuronal GABA transporters. The GAT-1 protein shares antigenic determinants with a native rat brain GABA transporter. The nucleotide sequence of GAT-1 predicts a protein of 599 amino acids with a molecular weight of 67 kilodaltons. Hydropathy analysis of the deduced protein suggests multiple transmembrane regions, a feature shared by several cloned transporters; however, database searches indicate that GAT-1 is not homologous to any previously identified proteins. Therefore, GAT-1 appears to be a member of a previously uncharacterized family of transport molecules.

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Lester, Henry A.0000-0002-5470-5255
Additional Information:© 1990 American Association for the Advancement of Science. 30 March 1990; Accepted 8 June 1990. We thank R. Radian and A. Bendahan for protein purification, and I. Ahmed, J. Hoger, D. Krafte, C. LaBarca, T. Snutch, and A. Walter for sharing protocols and expertise. We also thank T. Snutch for participating in the construction of the cDNA library, S. Celniker and the Caltech fly group for the use of their oligonucleotide synthesizer, and S. Bajjalieh for comments on the manuscript. We especially thank A. Gouin for oocyte preparation. Supported by NIH grants GM 29836, GM 10991, and NS 16708, by U.S.-Israel Binational Science Foundation grant 86-00147, and by an NIH postdoctoral fellowship to J.G.
Funding AgencyGrant Number
NIHGM 29836
NIHGM 10991
NIHNS 16708
Binational Science Foundation (USA-Israel)86-00147
NIH Postdoctoral FellowshipUNSPECIFIED
Issue or Number:4974
Record Number:CaltechAUTHORS:20141222-150126708
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Official Citation:Guastella, J., Nelson, N., Nelson, H., Czyzyk, L., Keynan, S., Miedel, M., . . . Kanner, B. (1990). Cloning and expression of a rat brain GABA transporter. Science, 249(4974), 1303-1306. doi: 10.1126/science.1975955
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53114
Deposited By: Joanne McCole
Deposited On:23 Dec 2014 17:06
Last Modified:10 Nov 2021 19:48

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