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A Ligase-Mediated Gene Detection Technique

Landegren, Ulf and Kaiser, Robert and Sanders, Jane and Hood, Leroy (1988) A Ligase-Mediated Gene Detection Technique. Science, 241 (4869). pp. 1077-1080. ISSN 0036-8075. http://resolver.caltech.edu/CaltechAUTHORS:20141222-153953460

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Abstract

An assay for the presence of given DNA sequences has been developed, based on the ability of two oligonucleotides to anneal immediately adjacent to each other on a complementary target DNA molecule. The two oligonucleotides are then joined covalently by the action of a DNA ligase, provided that the nucleotides at the junction are correctly base-paired. Thus single nucleotide substitutions can be distinguished. This strategy permits the rapid and standardized identification of single-copy gene sequences in genomic DNA.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1126/science.3413476DOIArticle
http://www.sciencemag.org/content/241/4869/1077PublisherArticle
http://www.jstor.org/stable/1702411JSTORArticle
Additional Information:© 1988 American Association for the Advancement of Science. Received 11 April 1988; accepted 23 June 1988. The authors acknowledge a stipend from the Knut and Alice Wallenberg Foundation to U.L. and support from NSF grant BNS 87 14486, Defense Advanced Research Projects Agency grant N00014-86K-0755, Upjohn Company, and Applied Biosystems, Inc. The oligonucleotides were synthesized by S. J. Horvath and the fluorescence data were analyzed by C. Dodd. R. K. Saiki provided plasmids and samples of genomic DNA obtained from cell lines. J. Korenberg and K. Tanaka made available blood samples from sickle cells patients. The Nhydroxysuccinimide ester of carboxy-2',7'-dimethoxy- 4',5'-dichlorofluorescein was provided by M. W. Hunkapiller. We acknowledge discussions with B. Korber, B. Popko, A. Kamb, N. Lan, L. Smith, R. Barth, V. A. McKusick, J. Richards, and M. Simon.
Funders:
Funding AgencyGrant Number
Knut and Alice Wallenberg FoundationUNSPECIFIED
NSFBNS 87 14486
Office of Naval Research (ONR)N00014-86K-0755
Upjohn CompanyUNSPECIFIED
Applied Biosystems, Inc.UNSPECIFIED
Defense Advanced Research Projects Agency (DARPA)UNSPECIFIED
Record Number:CaltechAUTHORS:20141222-153953460
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20141222-153953460
Official Citation:A ligase-mediated gene detection technique U Landegren, R Kaiser, J Sanders, and L Hood Science 26 August 1988: 241 (4869), 1077-1080. [DOI:10.1126/science.3413476]
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53120
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:23 Dec 2014 16:47
Last Modified:23 Dec 2014 16:47

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