Moser, Heinz E. and Dervan, Peter B. (1987) Sequence-specific cleavage of double helical DNA by triple helix formation. Science, 238 (4827). pp. 645-650. ISSN 0036-8075. doi:10.1126/science.3118463. https://resolver.caltech.edu/CaltechAUTHORS:20141223-091109973
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Abstract
Homopyrimidine oligodeoxyribonucleotides with EDTA-Fe attached at a single position bind the corresponding homopyrimidine-homopurine tracts within large double-stranded DNA by triple helix formation and cleave at that site. Oligonucleotides with EDTA.Fe at the 5' end cause a sequence specific double strand break. The location and asymmetry of the cleavage pattern reveal that the homopyrimidine-EDTA probes bind in the major groove parallel to the homopurine strand of Watson-Crick double helical DNA. The sequence-specific recognition of double helical DNA by homopyrimidine probes is sensitive to single base mismatches. Homopyrimidine probes equipped with DNA cleaving moieties could be useful tools for mapping chromosomes.
| Item Type: | Article | ||||||||||||
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| Additional Information: | © 1987 American Association for the Advancement of Science. Received 8 July 1987; accepted 21 September 1987. Supported by grants from the National Institutes of Health (GM 35724) and the Ralph M. Parsons Foundation, and a fellowship (to H.E.M.) from the Swiss National Science Foundation. | ||||||||||||
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| Issue or Number: | 4827 | ||||||||||||
| DOI: | 10.1126/science.3118463 | ||||||||||||
| Record Number: | CaltechAUTHORS:20141223-091109973 | ||||||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20141223-091109973 | ||||||||||||
| Official Citation: | Sequence-specific cleavage of double helical DNA by triple helix formation HE Moser and PB Dervan Science 30 October 1987: 238 (4827), 645-650. [DOI:10.1126/science.3118463] | ||||||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||||
| ID Code: | 53137 | ||||||||||||
| Collection: | CaltechAUTHORS | ||||||||||||
| Deposited By: | Tony Diaz | ||||||||||||
| Deposited On: | 23 Dec 2014 17:38 | ||||||||||||
| Last Modified: | 10 Nov 2021 19:48 |
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