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Sequence-specific cleavage of double helical DNA by triple helix formation

Moser, Heinz E. and Dervan, Peter B. (1987) Sequence-specific cleavage of double helical DNA by triple helix formation. Science, 238 (4827). pp. 645-650. ISSN 0036-8075. doi:10.1126/science.3118463.

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Homopyrimidine oligodeoxyribonucleotides with EDTA-Fe attached at a single position bind the corresponding homopyrimidine-homopurine tracts within large double-stranded DNA by triple helix formation and cleave at that site. Oligonucleotides with EDTA.Fe at the 5' end cause a sequence specific double strand break. The location and asymmetry of the cleavage pattern reveal that the homopyrimidine-EDTA probes bind in the major groove parallel to the homopurine strand of Watson-Crick double helical DNA. The sequence-specific recognition of double helical DNA by homopyrimidine probes is sensitive to single base mismatches. Homopyrimidine probes equipped with DNA cleaving moieties could be useful tools for mapping chromosomes.

Item Type:Article
Related URLs:
URLURL TypeDescription
Dervan, Peter B.0000-0001-8852-7306
Additional Information:© 1987 American Association for the Advancement of Science. Received 8 July 1987; accepted 21 September 1987. Supported by grants from the National Institutes of Health (GM 35724) and the Ralph M. Parsons Foundation, and a fellowship (to H.E.M.) from the Swiss National Science Foundation.
Funding AgencyGrant Number
NIHGM 35724
Ralph M. Parsons FoundationUNSPECIFIED
Swiss National Science Foundation (SNSF)UNSPECIFIED
Issue or Number:4827
Record Number:CaltechAUTHORS:20141223-091109973
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Official Citation:Sequence-specific cleavage of double helical DNA by triple helix formation HE Moser and PB Dervan Science 30 October 1987: 238 (4827), 645-650. [DOI:10.1126/science.3118463]
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53137
Deposited By: Tony Diaz
Deposited On:23 Dec 2014 17:38
Last Modified:10 Nov 2021 19:48

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