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Non-canonical amino acids in protein engineering

Link, A. James and Mock, Marissa L. and Tirrell, David A. (2003) Non-canonical amino acids in protein engineering. Current Opinion in Biotechnology, 14 (6). pp. 603-609. ISSN 0958-1669. doi:10.1016/j.copbio.2003.10.011.

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Methods for engineering proteins that contain non-canonical amino acids have advanced rapidly in the past few years. Novel amino acids can be introduced into recombinant proteins in either a residue-specific or site-specific fashion. The methods are complementary: residue-specific incorporation allows engineering of the overall physical and chemical behavior of proteins and protein-like macromolecules, whereas site-specific methods allow mechanistic questions to be probed in atomistic detail. Challenges remain in the engineering of the translational apparatus and in the design of schemes that can be used to encode both canonical and non-canonical amino acids.

Item Type:Article
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Tirrell, David A.0000-0003-3175-4596
Additional Information:© 2003 Elsevier. Work on non-canonical amino acids at Caltech has been supported by grants from the National Science Foundation, the National Institutes of Health and the US Army Research Office.
Funding AgencyGrant Number
Army Research Office (ARO)UNSPECIFIED
Subject Keywords:GFP, green fluorescent protein; nAChR, nicotinic acetylcholine receptor
Issue or Number:6
Record Number:CaltechAUTHORS:LINcob2003
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Official Citation:A James Link, Marissa L Mock, David A Tirrell, Non-canonical amino acids in protein engineering, Current Opinion in Biotechnology, Volume 14, Issue 6, December 2003, Pages 603-609, ISSN 0958-1669, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53311
Deposited By: Anne Hormann
Deposited On:07 Feb 2015 20:01
Last Modified:10 Nov 2021 20:00

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