CaltechAUTHORS
  A Caltech Library Service

Mechanical Properties of Artificial Protein Matrices Engineered for Control of Cell and Tissue Behavior

Di Zio, Kathleen and Tirrell, David A. (2003) Mechanical Properties of Artificial Protein Matrices Engineered for Control of Cell and Tissue Behavior. Macromolecules, 36 (5). pp. 1553-1558. ISSN 0024-9297. doi:10.1021/ma0256587. https://resolver.caltech.edu/CaltechAUTHORS:ZIOm2003

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:ZIOm2003

Abstract

Genetic engineering methods were used for the preparation of artificial proteins containing sequences designed to reproduce essential features of the extracellular matrix (ECM). The long-term objective of the work is to develop matrices for use in the engineering of small-diameter vascular grafts. The CS5 domain of fibronectin provides binding sites for vascular endothelial cells, while an elastin-like repeat, [(VPGIG)_2(VPGKG)(VPGIG)_2], controls the mechanical properties and includes sites for covalent cross-linking. Bis(sulfosuccinimidyl) suberate and disuccinimidyl suberate were used to cross-link artificial ECM protein films for uniaxial tensile testing. Variation in the amount of cross-linker and protein weight fraction allowed preparation of films with Young's moduli ranging from 0.07 to 0.97 MPa. The weight fraction of protein in the hydrated, cross-linked films was measured to be between 0.2 and 0.4; the molecular weight between cross-links (M_c) varied from 3000 to 38 000. The moduli and M_c of the films span the ranges reported for natural elastins.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ma0256587DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ma0256587PublisherArticle
ORCID:
AuthorORCID
Tirrell, David A.0000-0003-3175-4596
Additional Information:Copyright © 2003 American Chemical Society. Received September 9, 2002; Revised Manuscript Received January 2, 2003. Publication Date (Web): February 14, 2003. This work was supported by NIH Grant 5 RO1 HL59987-03 and by NSF Grant BES-9901648. Mass spectra were recorded in the central facility for mass spectrometry of the Center for the Science and Engineering of Materials at Caltech.
Funders:
Funding AgencyGrant Number
NIH5 RO1 HL59987-03
NSFBES-9901648
Issue or Number:5
DOI:10.1021/ma0256587
Record Number:CaltechAUTHORS:ZIOm2003
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:ZIOm2003
Official Citation:Mechanical Properties of Artificial Protein Matrices Engineered for Control of Cell and Tissue Behavior Kathleen Di Zio and David A. Tirrell Macromolecules 2003 36 (5), 1553-1558
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53341
Collection:CaltechAUTHORS
Deposited By: Anne Hormann
Deposited On:07 Feb 2015 01:14
Last Modified:10 Nov 2021 20:00

Repository Staff Only: item control page