Di Zio, Kathleen and Tirrell, David A. (2003) Mechanical Properties of Artificial Protein Matrices Engineered for Control of Cell and Tissue Behavior. Macromolecules, 36 (5). pp. 1553-1558. ISSN 0024-9297. doi:10.1021/ma0256587. https://resolver.caltech.edu/CaltechAUTHORS:ZIOm2003
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Abstract
Genetic engineering methods were used for the preparation of artificial proteins containing sequences designed to reproduce essential features of the extracellular matrix (ECM). The long-term objective of the work is to develop matrices for use in the engineering of small-diameter vascular grafts. The CS5 domain of fibronectin provides binding sites for vascular endothelial cells, while an elastin-like repeat, [(VPGIG)_2(VPGKG)(VPGIG)_2], controls the mechanical properties and includes sites for covalent cross-linking. Bis(sulfosuccinimidyl) suberate and disuccinimidyl suberate were used to cross-link artificial ECM protein films for uniaxial tensile testing. Variation in the amount of cross-linker and protein weight fraction allowed preparation of films with Young's moduli ranging from 0.07 to 0.97 MPa. The weight fraction of protein in the hydrated, cross-linked films was measured to be between 0.2 and 0.4; the molecular weight between cross-links (M_c) varied from 3000 to 38 000. The moduli and M_c of the films span the ranges reported for natural elastins.
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| Additional Information: | Copyright © 2003 American Chemical Society. Received September 9, 2002; Revised Manuscript Received January 2, 2003. Publication Date (Web): February 14, 2003. This work was supported by NIH Grant 5 RO1 HL59987-03 and by NSF Grant BES-9901648. Mass spectra were recorded in the central facility for mass spectrometry of the Center for the Science and Engineering of Materials at Caltech. | |||||||||
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| Issue or Number: | 5 | |||||||||
| DOI: | 10.1021/ma0256587 | |||||||||
| Record Number: | CaltechAUTHORS:ZIOm2003 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:ZIOm2003 | |||||||||
| Official Citation: | Mechanical Properties of Artificial Protein Matrices Engineered for Control of Cell and Tissue Behavior Kathleen Di Zio and David A. Tirrell Macromolecules 2003 36 (5), 1553-1558 | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 53341 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Anne Hormann | |||||||||
| Deposited On: | 07 Feb 2015 01:14 | |||||||||
| Last Modified: | 10 Nov 2021 20:00 |
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