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Identification of an expanded set of translationally active methionine analogues in Escherichia coli

Kiick, K. L. and Weberskirch, R. and Tirrell, D. A. (2001) Identification of an expanded set of translationally active methionine analogues in Escherichia coli. FEBS Letters, 502 (1-2). pp. 25-30. ISSN 0014-5793 . https://resolver.caltech.edu/CaltechAUTHORS:KIIfl2001

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Abstract

Amino acid incorporation into proteins in vivo is controlled most stringently by the aminoacyl-tRNA synthetases. Here we report the incorporation of several new methionine analogues into protein by increasing the rate of their activation by the methionyl-tRNA synthetase (MetRS) of Escherichia coli. cis-Crotylglycine (4), 2-aminoheptanoic acid (7), norvaline (8), 2-butynylglycine (11), and allylglycine (12) will each support protein synthesis in methionine-depleted cultures of E. coli when MetRS is overexpressed and the medium is supplemented with the analogue at millimolar concentrations. These investigations suggest important opportunities for protein engineering, as expansion of the translational apparatus toward other amino acid analogues by similar strategies should also be possible.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/S0014-5793(01)02657-6DOIArticle
http://www.sciencedirect.com/science/article/pii/S0014579301026576PublisherArticle
http://dx.doi.org/10.1016/S0014-5793(01)02857-5DOIErratum
http://www.sciencedirect.com/science/article/pii/S0014579301028575PublisherErratum
ORCID:
AuthorORCID
Tirrell, D. A.0000-0003-3175-4596
Additional Information:© 2001 Elsevier. Received 6 June 2001; accepted 13 June 2001. First published online 9 July 2001. This work was supported by grants from the Polymers and Genetics Programs of the U.S. National Science Foundation and from the U.S. Army Research Office. We are grateful to J.C.M. van Hest and to H. Blackwell for synthesis of methionine analogues and to H. Jakubowski and Y. Mechulam for donation of plasmids encoding MetRS. K.L.K. thanks the U.S. Department of Defense for a National Defense Science and Engineering Graduate Fellowship.
Errata:Corrigendum to: Identification of an expanded set of translationally active methionine analogues in Escherichia coli (FEBS 25068): [FEBS Letters 502 (2001) 25–30] K.L. Kiick, R. Weberskirch, D.A. Tirrell
Funders:
Funding AgencyGrant Number
NSFUNSPECIFIED
Army Research Office (ARO)UNSPECIFIED
National Defense Science and Engineering Graduate (NDSEG) FellowshipUNSPECIFIED
Subject Keywords:Methionyl-tRNA synthetase; Protein engineering; Methionine; Non-natural amino acid
Issue or Number:1-2
Record Number:CaltechAUTHORS:KIIfl2001
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:KIIfl2001
Official Citation:K.L. Kiick, R. Weberskirch, D.A. Tirrell, Identification of an expanded set of translationally active methionine analogues in Escherichia coli, FEBS Letters, Volume 502, Issues 1–2, 27 July 2001, Pages 25-30, ISSN 0014-5793, http://dx.doi.org/10.1016/S0014-5793(01)02657-6. (http://www.sciencedirect.com/science/article/pii/S0014579301026576)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53353
Collection:CaltechAUTHORS
Deposited By: Anne Hormann
Deposited On:07 Feb 2015 18:24
Last Modified:03 Oct 2019 07:49

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