CaltechAUTHORS
  A Caltech Library Service

Dynamic Structure of a Protein Hydrogel: A Solid-State NMR Study

Kennedy, S. B. and deAzevedo, E. R. and Petka, W. A. and Russell, T. P. and Tirrell, D. A. and Hong, M. (2001) Dynamic Structure of a Protein Hydrogel: A Solid-State NMR Study. Macromolecules, 34 (25). pp. 8675-8685. ISSN 0024-9297. https://resolver.caltech.edu/CaltechAUTHORS:KENm2001

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:KENm2001

Abstract

^(13)C and ^(15)N solid-state NMR spectroscopy has been used to study the dynamic structure of a genetically engineered multidomain protein hydrogel that contains two leucine-zipper domains and a central polyelectrolyte domain. ^(13)C NMR spectra show that on the microsecond time scale the central domain is isotropically mobile while the leucine-zipper domains are rigid. This supports the hypothesis that the central domain acts as the flexible swelling agent of the gel network while the terminal domains form intermolecular aggregates. ^(13)C isotropic chemical shifts indicate that the terminal domains are helical, while the central domain has a random coil conformation. On the millisecond time scale, the leucine-zipper domains are highly dynamic, as determined from the ^(13)C-detected ^(15)N CODEX experiment. The motion is rigid-body in nature with a correlation time of about 80 ms at room temperature and has an average amplitude of about 50°. Several specific motional models are considered by comparing simulated and experimental exchange intensities as a function of the recoupling time for ^(15)N chemical shift anisotropy. The experimental data are consistent with two of the models considered:  a random jump model and a uniaxial rotation model. The implications of this motion to strand exchange between helical bundles are discussed.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ma010768jDOIArticle
http://pubs.acs.org/doi/abs/10.1021/ma010768jPublisherArticle
ORCID:
AuthorORCID
Tirrell, D. A.0000-0003-3175-4596
Additional Information:Copyright © 2001 American Chemical Society. Received May 4, 2001. Publication Date (Web): November 7, 2001. This work was partially supported by the NSF Materials Science and Engineering Center at the University of Massachusetts, Amherst. M. Hong acknowledges NSF for a POWRE award and the Beckman Foundation for a Young Investigator Award. E. R. deAzevedo thanks the FAPESP-Brazil program for a graduate fellowship.
Funders:
Funding AgencyGrant Number
NSFUNSPECIFIED
Arnold and Mabel Beckman FoundationUNSPECIFIED
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)UNSPECIFIED
Issue or Number:25
Record Number:CaltechAUTHORS:KENm2001
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:KENm2001
Official Citation:Dynamic Structure of a Protein Hydrogel:  A Solid-State NMR Study S. B. Kennedy, E. R. deAzevedo, W. A. Petka, and, T. P. Russell, D. A. Tirrell, and M. Hong Macromolecules 2001 34 (25), 8675-8685
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53355
Collection:CaltechAUTHORS
Deposited By: Anne Hormann
Deposited On:07 Feb 2015 01:31
Last Modified:03 Oct 2019 07:49

Repository Staff Only: item control page