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Reversible Hydrogels from Self-Assembling Artificial Proteins

Petka, Wendy A. and Harden, James L. and McGrath, Kevin P. and Wirtz, Denis and Tirrell, David A. (1998) Reversible Hydrogels from Self-Assembling Artificial Proteins. Science, 281 (5375). pp. 389-392. ISSN 0036-8075. https://resolver.caltech.edu/CaltechAUTHORS:PETs1998

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Abstract

Recombinant DNA methods were used to create artificial proteins that undergo reversible gelation in response to changes in pH or temperature. The proteins consist of terminal leucine zipper domains flanking a central, flexible, water-soluble polyelectrolyte segment. Formation of coiled-coil aggregates of the terminal domains in near-neutral aqueous solutions triggers formation of a three-dimensional polymer network, with the polyelectrolyte segment retaining solvent and preventing precipitation of the chain. Dissociation of the coiled-coil aggregates through elevation of pH or temperature causes dissolution of the gel and a return to the viscous behavior that is characteristic of polymer solutions. The mild conditions under which gel formation can be controlled (near-neutral pH and near-ambient temperature) suggest that these materials have potential in bioengineering applications requiring encapsulation or controlled release of molecular and cellular species.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1126/science.281.5375.389DOIArticle
http://www.sciencemag.org/content/281/5375/389PublisherArticle
ORCID:
AuthorORCID
Tirrell, David A.0000-0003-3175-4596
Additional Information:© 1998 American Association for the Advancement of Science. 5 February 1998; accepted 3 June 1998. Supported by grants from NSF (to D.W. and D.A.T.), the Whitaker Foundation (to D.W.), and the U.S. Army Natick Research Development and Engineering Center (to D.A.T.). We thank K. Rufener and V. Viasnoff for useful discussions and assistance.
Funders:
Funding AgencyGrant Number
NSFUNSPECIFIED
Whitaker FoundationUNSPECIFIED
U.S. Army Natick Research Development and Engineering CenterUNSPECIFIED
Issue or Number:5375
Record Number:CaltechAUTHORS:PETs1998
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:PETs1998
Official Citation:Reversible Hydrogels from Self-Assembling Artificial Proteins Wendy A. Petka, James L. Harden, Kevin P. McGrath, Denis Wirtz, and David A. Tirrell Science 17 July 1998: 281 (5375), 389-392. [DOI:10.1126/science.281.5375.389]
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53392
Collection:CaltechAUTHORS
Deposited By: Anne Hormann
Deposited On:06 Feb 2015 04:49
Last Modified:03 Oct 2019 07:49

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