Petka, Wendy A. and Harden, James L. and McGrath, Kevin P. and Wirtz, Denis and Tirrell, David A. (1998) Reversible Hydrogels from Self-Assembling Artificial Proteins. Science, 281 (5375). pp. 389-392. ISSN 0036-8075. https://resolver.caltech.edu/CaltechAUTHORS:PETs1998
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Abstract
Recombinant DNA methods were used to create artificial proteins that undergo reversible gelation in response to changes in pH or temperature. The proteins consist of terminal leucine zipper domains flanking a central, flexible, water-soluble polyelectrolyte segment. Formation of coiled-coil aggregates of the terminal domains in near-neutral aqueous solutions triggers formation of a three-dimensional polymer network, with the polyelectrolyte segment retaining solvent and preventing precipitation of the chain. Dissociation of the coiled-coil aggregates through elevation of pH or temperature causes dissolution of the gel and a return to the viscous behavior that is characteristic of polymer solutions. The mild conditions under which gel formation can be controlled (near-neutral pH and near-ambient temperature) suggest that these materials have potential in bioengineering applications requiring encapsulation or controlled release of molecular and cellular species.
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| Additional Information: | © 1998 American Association for the Advancement of Science. 5 February 1998; accepted 3 June 1998. Supported by grants from NSF (to D.W. and D.A.T.), the Whitaker Foundation (to D.W.), and the U.S. Army Natick Research Development and Engineering Center (to D.A.T.). We thank K. Rufener and V. Viasnoff for useful discussions and assistance. | |||||||||
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| Issue or Number: | 5375 | |||||||||
| Record Number: | CaltechAUTHORS:PETs1998 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:PETs1998 | |||||||||
| Official Citation: | Reversible Hydrogels from Self-Assembling Artificial Proteins Wendy A. Petka, James L. Harden, Kevin P. McGrath, Denis Wirtz, and David A. Tirrell Science 17 July 1998: 281 (5375), 389-392. [DOI:10.1126/science.281.5375.389] | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 53392 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Anne Hormann | |||||||||
| Deposited On: | 06 Feb 2015 04:49 | |||||||||
| Last Modified: | 03 Oct 2019 07:49 |
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