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Structure of a Thioredoxin-like [2Fe-2S] Ferredoxin from Aquifex aeolicus

Yeh, Andrew P. and Chatelet, Claire and Soltis, S. Michael and Kuhn, Peter and Meyer, Jacques and Rees, Douglas C. (2000) Structure of a Thioredoxin-like [2Fe-2S] Ferredoxin from Aquifex aeolicus. Journal of Molecular Biology, 300 (3). pp. 587-595. ISSN 0022-2836. http://resolver.caltech.edu/CaltechAUTHORS:20150108-154426156

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Abstract

The 2.3 Å resolution crystal structure of a [2Fe-2S] cluster containing ferredoxin from Aquifex aeolicusreveals a thioredoxin-like fold that is novel among iron-sulfur proteins. The [2Fe-2S] cluster is located near the surface of the protein, at a site corresponding to that of the active-site disulfide bridge in thioredoxin. The four cysteine ligands are located near the ends of two surface loops. Two of these ligands can be substituted by non-native cysteine residues introduced throughout a stretch of the polypeptide chain that forms a protruding loop extending away from the cluster. The presence of homologs of this ferredoxin as components of more complex anaerobic and aerobic electron transfer systems indicates that this is a versatile fold for biological redox processes.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1006/jmbi.2000.3871 DOIArticle
http://www.sciencedirect.com/science/article/pii/S0022283600938713PublisherArticle
Contact Email Address:phoebe@caltech.edu
Additional Information:© 2000 Academic Press. Received 29 March 2000; received in revised form 12 May 2000; accepted 15 May 2000. We thank the National Institutes of Health (D.C.R.) for support. A.P.Y. was supported in part by a National Science Foundation Graduate Research Fellowship. This work is based upon research conducted at the Stanford Synchrotron Radiation Laboratory (SSRL), which is funded by the Department of Energy, Office of Basic Energy Sciences. The Biotechnology Program is supported by the National Institutes of Health, National Center for Research Resources, Biomedical Technology Program and the Department of Energy, Office of Biological and Environmental Research.
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Funding AgencyGrant Number
NIHUNSPECIFIED
NSF Graduate Research FellowshipUNSPECIFIED
Department of Energy (DOE)UNSPECIFIED
Subject Keywords:crystal structure; [2Fe-2S] ferredoxin; thioredoxin; respiratory chain; iron-sulfur clusters
Record Number:CaltechAUTHORS:20150108-154426156
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20150108-154426156
Official Citation:Andrew P Yeh, Claire Chatelet, S.Michael Soltis, Peter Kuhn, Jacques Meyer, Douglas C Rees, Structure of a thioredoxin-like [2Fe-2S] ferredoxin from Aquifex aeolicus, Journal of Molecular Biology, Volume 300, Issue 3, 14 July 2000, Pages 587-595, ISSN 0022-2836, http://dx.doi.org/10.1006/jmbi.2000.3871. (http://www.sciencedirect.com/science/article/pii/S0022283600938713)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53413
Collection:CaltechAUTHORS
Deposited By: SWORD User
Deposited On:14 Jan 2015 07:29
Last Modified:14 Jan 2015 07:29

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