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Comparing Crystallographic and Solution Structures of Nitrogenase Complexes

Grossmann, J. Günter and Hasnain, S. Samar and Yousafzai, Faridoon K. and Smith, Barry E. and Eady, Robert R. and Schindelin, Hermann and Kisker, Caroline and Howard, James B. and Tsuruta, Hirotsugu and Muller, Jurgen and Rees, Douglas C. (1999) Comparing Crystallographic and Solution Structures of Nitrogenase Complexes. Acta Crystallographica Section D: Biological Crystallography, 55 (4). pp. 727-728. ISSN 0907-4449. doi:10.1107/S0907444999003856.

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A low-resolution structure from X-ray scattering data of Kp1•(ADP•AlF-_4•Kp2)2 predicted a significant change in the iron protein (Kp2) upon complex formation. This has been subsequently confirmed by the crystallographic structure of the complex in the Av system. New scattering results are provided to demonstrate the similarity of this complex in the two species.

Item Type:Article
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URLURL TypeDescription DOIArticle
Rees, Douglas C.0000-0003-4073-1185
Additional Information:© 1999 International Union of Crystallography. The work was supported by the EPSRC/BBSRC Biomolecular Science grant GR/J54512 and by a grant from the NIH. The small-angle scattering facility at SSRL is supported by DOE and NIH. We are also grateful for the provision of facilities at CCLRC Daresbury Laboratory and to Dr Loretta Murphy for her help and interest. We acknowledge helpful comments from the referees.
Funding AgencyGrant Number
Engineering and Physical Sciences Research Council (EPSRC)GR/J54512
Biotechnology and Biological Sciences Research Council (BBSRC)UNSPECIFIED
Department of Energy (DOE)UNSPECIFIED
Subject Keywords:small-angle X-ray scattering; protein-protein interactions; nitrogenase complexes
Issue or Number:4
Record Number:CaltechAUTHORS:20150108-155917509
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Official Citation:Grossmann, J. G., Hasnain, S. S., Yousafzai, F. K., Smith, B. E., Eady, R. R., Schindelin, H., Kisker, C., Howard, J. B., Tsuruta, H., Muller, J. & Rees, D. C. (1999). Acta Cryst. D55, 727-728.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53423
Deposited By: SWORD User
Deposited On:13 Jan 2015 16:58
Last Modified:10 Nov 2021 20:01

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