Kielkopf, Clara L. and Baird, Eldon E. and Dervan, Peter B. and Rees, Douglas C. (1998) Structural Basis for G•C Recognition in the DNA Minor Groove. Nature Structural Biology, 5 (2). pp. 104-109. ISSN 1072-8368. doi:10.1038/nsb0298-104. https://resolver.caltech.edu/CaltechAUTHORS:20150108-160953214
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Abstract
Small molecules that target specific DNA sequences offer a potentially general approach for the regulation of gene expression. Pyrrole−imidazole polyamides represent the only class of synthetic small molecules that can bind predetermined DNA sequences with affinities and specificities comparable to DNA binding proteins. Antiparallel side-by-side pairings of two aromatic amino acids, imidazole (Im) and pyrrole (Py), distinguish G•C from C•G, and both from A•T/T•A base pairs. A high resolution X-ray crystal structure of a four-ring pyrrole−imidazole polyamide specifically bound as a dimer to a six-base pair predetermined DNA site reveals a structural framework of hydrogen bonds and interactions with the walls of the minor groove that underlies the pairing rules for DNA recognition.
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Additional Information: | © 1998 Nature Publishing Group. Received 3 November, 1997; accepted 30 December, 1997. We are grateful to the NIH for research support, the NSF for a predoctoral fellowship to C.L.K., and the HHMI for a predoctoral fellowship to E.E.B. We thank JE. Wedekind, H. Schindelin, C. Kisker, L. Joshua-Tor, PM. Takahara and the members of the Rees group for guidance during the structure determination. The rotation camera facility at SSRL is supported by the Department of Energy and NIH. | ||||||||||
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Issue or Number: | 2 | ||||||||||
DOI: | 10.1038/nsb0298-104 | ||||||||||
Record Number: | CaltechAUTHORS:20150108-160953214 | ||||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20150108-160953214 | ||||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||
ID Code: | 53430 | ||||||||||
Collection: | CaltechAUTHORS | ||||||||||
Deposited By: | SWORD User | ||||||||||
Deposited On: | 09 Jan 2015 19:04 | ||||||||||
Last Modified: | 10 Nov 2021 20:01 |
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