Deming, Timothy J. and Fournier, Maurille J. and Mason, Thomas L. and Tirrell, David A. (1997) Biosynthetic Incorporation and Chemical Modification of Alkene Functionality in Genetically Engineered Polymers. Journal of Macromolecular Science, Part A, 34 (10). pp. 2143-2150. ISSN 1060-1325. doi:10.1080/10601329708010331. https://resolver.caltech.edu/CaltechAUTHORS:DEMjmsa1997
Full text is not posted in this repository. Consult Related URLs below.
Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:DEMjmsa1997
Abstract
Repetitive polypeptides of sequence [(AlaGly)_3ProGluGly]_(16) 3a, have been prepared in Escherichia coli as overexpressed recombinant proteins. Replacement of more than 90% of the naturally occurring proline (Pro) residues with 3,4-dehydroproline (Dhp) in sequence 3a was achieved by in vivo expression of the target protein in medium containing Dhp and lacking Pro. The resulting material (3b) was treated with H_2O_2 or Br_2 to yield polymers containing 3,4-dihydroxyproline (Dhy, 3c) and 3,4-dibromoproline (Dbr, 3d), respectively, in place of the Dhp residue. These results represent the first demonstration of the incorporation and modification of alkene functionality in recombinant proteins.
| Item Type: | Article | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Related URLs: |
| |||||||||
| ORCID: |
| |||||||||
| Additional Information: | © 1997 Marcel Dekker. This work was supported by Contract No. DAAH04-93-G-0217 from the U. S. Army Research Office and by a grant (DMR-8914359) from the Polymers and Genetics Programs of the National Science Foundation. T. J. D. acknowledges the National Institutes of Health for a National Research Service Award postdoctoral training fellowship. NMR spectra were recorded in the University of Massachusetts NMR Facility, which is supported in part by the NSF Materials Research Science and Engineering Center at the University. | |||||||||
| Funders: |
| |||||||||
| Issue or Number: | 10 | |||||||||
| DOI: | 10.1080/10601329708010331 | |||||||||
| Record Number: | CaltechAUTHORS:DEMjmsa1997 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:DEMjmsa1997 | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 53440 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Anne Hormann | |||||||||
| Deposited On: | 31 Jan 2015 23:29 | |||||||||
| Last Modified: | 10 Nov 2021 20:02 |
Repository Staff Only: item control page
