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Crystalline Aggregates of the Repetitive Polypeptide {(AlaGly)_3GluGly(GlyAla)_3GluGly}_(10): Structure and Dynamics Probed by ^(13)C Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy

Wang, Jianxin and Parkhe, Ajay D. and Tirrell, David A. and Thompson , Lynmarie K. (1996) Crystalline Aggregates of the Repetitive Polypeptide {(AlaGly)_3GluGly(GlyAla)_3GluGly}_(10): Structure and Dynamics Probed by ^(13)C Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy. Macromolecules, 29 (5). pp. 1548-1553. ISSN 0024-9297. http://resolver.caltech.edu/CaltechAUTHORS:WANm1996

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Abstract

A repetitive polymer comprising 10 repeats of the oligopeptide (AlaGly)_3GluGly(GlyAla)_3 GluGly has been synthesized via bacterial expression of an artificial gene. This polymer was designed to assemble into lamellar crystals of predictable thickness, with the oligo(AlaGly) and oligo(GlyAla) portions forming the crystal stems and the polar, bulky Glu residues lying in reverse turns between adjacent, antiparallel β-strands. The solid-state structure and dynamics of this material, before and after crystallization, have been probed with a range of magic angle spinning ^(13)C NMR experiments. Chemical shifts of backbone carbons indicate that the oligo(AlaGly) elements adopt β-conformations in all samples. Changes in both the chemical shift and cross-polarization behavior of the alanine side chains upon crystallization provide evidence for location of alanine within the crystalline β-sheet regions. In contrast, although crystallization induces changes in the chemical shift of C_α of the glutamic acid residue, no change occurs in the dynamic behavior of the Glu side chain carbons. The latter observation suggests that this side chain is excluded from the crystalline region and supports the assignment of Glu to turn positions at the lamellar surface.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ma9512972DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ma9512972PublisherArticle
ORCID:
AuthorORCID
Tirrell, David A.0000-0003-3175-4596
Alternate Title:Crystalline Aggregates of the Repetitive Polypeptide {(AlaGly)3GluGly(GlyAla)3GluGly}10:  Structure and Dynamics Probed by 13C Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy
Additional Information:Copyright © 1996 American Chemical Society. Received August 31, 1995; Revised Manuscript Received November 17, 1995. Publication Date (Web): February 26, 1996. Abstract published in Advance ACS Abstracts, February 1, 1996. This research was supported by grants from the NSF Materials Science and Engineering Center at the University of Massachusetts, an award from Research Corporation (L.K.T.), and an NSF Young Investigator Award (L.K.T.). The NMR instrument was purchased with a grant from NSF (BIR-911996) and matching funds from the University of Massachusetts. The authors acknowledge Drs. Charles Dickinson and Alan Waddon for helpful discussions.
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Funding AgencyGrant Number
NSFUNSPECIFIED
Research CorporationUNSPECIFIED
NSFBIR-911996
University of MassachusettsUNSPECIFIED
Record Number:CaltechAUTHORS:WANm1996
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:WANm1996
Official Citation:Crystalline Aggregates of the Repetitive Polypeptide {(AlaGly)3GluGly(GlyAla)3GluGly}10:  Structure and Dynamics Probed by 13C Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy Jianxin Wang, Ajay D. Parkhe, David A. Tirrell, and Lynmarie K. Thompson Macromolecules 1996 29 (5), 1548-1553
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53469
Collection:CaltechAUTHORS
Deposited By: Anne Hormann
Deposited On:29 Jan 2015 22:31
Last Modified:23 Apr 2019 20:52

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