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Modulation of Mobilities of Fluorescent Membrane Probes by Adsorption of a Hydrophobic Polyelectrolyte

Thomas, James L. and Borden, Keith A. and Tirrell, David A. (1996) Modulation of Mobilities of Fluorescent Membrane Probes by Adsorption of a Hydrophobic Polyelectrolyte. Macromolecules, 29 (7). pp. 2570-2576. ISSN 0024-9297. doi:10.1021/ma950855+.

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We have used three different fluorescence methods to examine the effect of the pH-dependent adsorption of a hydrophobic polyelectrolyte, poly(2-ethylacrylic acid), on the mobility of fluorescent probes in phosphatidylcholine membranes. Measurements of lateral diffusion, made by either pyrene excimer formation or fluorescence photobleaching recovery, show reduced probe mobility on polymer adsorption; formation of intramolecular excimers by 1,1‘-propylenebis(pyrene) is also reduced. Fluorescence depolarization measurements, however, show increased rotational freedom for diphenylhexatriene and other depolarization probes. Extensive polymer adsorption solubilizes membranes into micelles, but mobilities do not exhibit sharp transitions upon membrane reorganization. Polymer adsorption appears to affect probe mobilities locally; the global structure of the membrane does not play a direct role.

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Tirrell, David A.0000-0003-3175-4596
Additional Information:Copyright © 1996 American Chemical Society. Received June 16, 1995; Revised Manuscript Received January 2, 1996. Publication Date (Web): March 25, 1996. Abstract published in Advance ACS Abstracts, March 1, 1996. This work was supported by the NSF Materials Research Science and Engineering Center and the Center for Environmentally Appropriate Materials at the University of Massachusetts and by the Developmental Resource for Biological Imaging and Optoelectronics at Cornell University. We wish to thank Dr. David Piston for assistance in photobleaching measurements.
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Center for Environmentally Appropriate Materials, University of MassachusettsUNSPECIFIED
Cornell University, Developmental Resource for Biological Imaging and OptoelectronicsUNSPECIFIED
Issue or Number:7
Record Number:CaltechAUTHORS:THOm1996
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Official Citation:Modulation of Mobilities of Fluorescent Membrane Probes by Adsorption of a Hydrophobic Polyelectrolyte James L. Thomas, Keith A. Borden, and, and David A. Tirrell Macromolecules 1996 29 (7), 2570-2576
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53472
Deposited By: Anne Hormann
Deposited On:29 Jan 2015 22:17
Last Modified:10 Nov 2021 20:02

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