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Effect of Water on the Structure of a Model Polypeptide

Chen, C. C. and Krejchi, M. T. and Tirrell, D. A. and Hsu, S. L. (1995) Effect of Water on the Structure of a Model Polypeptide. Macromolecules, 28 (5). pp. 1464-1469. ISSN 0024-9297. doi:10.1021/ma00109a017.

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Vibrational spectroscopy in conjunction with X-ray and gravimetric methods has been used to study the structural stability of a hydrated polypeptide prepared in a genetically engineered strain of Escherichia coli. The sample adopts a β-sheet conformation in the solid state with a well-defined crystalline stem length and fold surfaces believed to be decorated with carboxylic acid groups. Ionization and subsequent hydration of these acid groups are found to have a major effect on the crystal packing and chain conformation. We have also established that the structural changes accompanying hydration of this model polypeptide occur in a stepwise fashion. First, because of their high accessibility, the carboxylic acid or carboxylate groups on the lamellar surfaces can readily interact with water molecules. In the second step, water penetrates into the regions between the hydrogen-bonded sheets; however, the resulting expansion in the intersheet distance can occur without altering the chain conformation. Lastly, when the water content is high, the hydrogen-bonded sheets are disrupted, leading to a change in chain conformation from ,&strands to helical or disordered chains.

Item Type:Article
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Tirrell, D. A.0000-0003-3175-4596
Additional Information:© 1995 American Chemical Society. Received August 26, 1994; Revised Manuscript Received November 29, 1994. Abstract published in Advance ACS Abstracts, February 1, 1995. This work was supported by the National Science Foundation Materials Research Science and Engineering Center at the University of Massachusetts and a grant from the Center for Environmentally Appropriate Materials. The authors would also like to thank Dr. N. S. Murthy of Allied-Signal for helpful discussions on the effect of water on semicrystalline polymers and Dr. A. D. Parkhe for discussions on the structure of the model polypeptide.
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Center for Environmentally Appropriate Materials, University of MassachusettsUNSPECIFIED
Issue or Number:5
Record Number:CaltechAUTHORS:CHEm1995
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Official Citation:Effect of Water on the Structure of a Model Polypeptide C. C. Chen, M. T. Krejchi, D. A. Tirrell, and S. L. Hsu Macromolecules 1995 28 (5), 1464-1469
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53480
Deposited By: Anne Hormann
Deposited On:29 Jan 2015 21:52
Last Modified:10 Nov 2021 20:02

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