CaltechAUTHORS
  A Caltech Library Service

Genetically Engineered Fluoropolymers. Synthesis of Repetitive Polypeptides Containing p-Fluorophenylalanine residues

Yoshikawa, Eiichiro and Fournier, Maurille J. and Mason, Thomas L. and Tirrell, David A. (1994) Genetically Engineered Fluoropolymers. Synthesis of Repetitive Polypeptides Containing p-Fluorophenylalanine residues. Macromolecules, 27 (19). pp. 5471-5475. ISSN 0024-9297. https://resolver.caltech.edu/CaltechAUTHORS:YOSm1994

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:YOSm1994

Abstract

Genetic engineering offers the potential for creating new proteins with novel materials properties. The aim of this study is to develop strategies for incorporating the non-natural amino acid p-fluorophenylalanine (pfF), which is known to be utilized by the Escherichia coli protein synthesis apparatus, into repetitive polypeptides. pfF has been successfully incorporated into target protein by in vivo substitution of pfF for phenylalanine in a phenylalanine auxotroph of the E. coli BL21(DE3) expression strain containing a chromosomal copy of the bacteriophage T7 RNA polymerase gene under LacUV5 promoter control. The auxotroph was generated by transposon mutagenesis using P1-mediated transduction. The repetitive protein -[(Ala.Gly)_3•pfF•Gly]_13- (1) was expressed from an artificial gene under T7 promoter control. Expression of 1 was carried out by shifting the host culture to pfF-containing medium after induction of T7 RNA polymerase for 10 min with isopropyl β-D-thiogalactopyranoside in medium containing the 20 natural amino acids. Replacement of 95-100% of phenylalanine by pfF was confirmed by ^1H NMR spectroscopy and amino acid analysis. Fourier transform infrared spectroscopy and X-ray scattering suggest that both 1 and the phenylalanine variant (2) adopt antiparallel β-sheet structures in the solid state.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ma00097a029DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ma00097a029PublisherArticle
ORCID:
AuthorORCID
Tirrell, David A.0000-0003-3175-4596
Additional Information:© 1994 American Chemical Society. Received April 1, 1994; Revised Manuscript Received June 22, 1994. Abstract published in Advance ACS Abstracts, August 15, 1994. This work was supported by the NSF Materials Research Laboratory at the University of Massachusetts and by a grant from the U.S. Army Research Office. We thank Kobe Steel, Ltd., for a leave of absence granted to E.Y.
Funders:
Funding AgencyGrant Number
NSFUNSPECIFIED
Army Research Office (ARO)UNSPECIFIED
Issue or Number:19
Record Number:CaltechAUTHORS:YOSm1994
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:YOSm1994
Official Citation:Genetically Engineered Fluoropolymers. Synthesis of Repetitive Polypeptides Containing p-Fluorophenylalanine residues Eiichiro Yoshikawa, Maurille J. Fournier, Thomas L. Mason, and David A. Tirrell Macromolecules 1994 27 (19), 5471-5475
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53487
Collection:CaltechAUTHORS
Deposited By: Anne Hormann
Deposited On:28 Jan 2015 20:48
Last Modified:03 Oct 2019 07:50

Repository Staff Only: item control page