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Chemical sequence control of β-sheet assembly in macromolecular crystals of periodic polypeptides

Krejchi, Mark T. and Atkins, Edward D. T. and Waddon, Alan J. and Fournier, Maurille J. and Mason, Thomas L. and Tirrell, David A. (1994) Chemical sequence control of β-sheet assembly in macromolecular crystals of periodic polypeptides. Science, 265 (5177). pp. 1427-1432. ISSN 0036-8075. https://resolver.caltech.edu/CaltechAUTHORS:KREs1994

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Abstract

A family of uniform periodic. polypeptides has been prepared by bacterial expression of the corresponding artificial genes, with the objective of exploring the potential for control of supramolecular organization in genetically engineered protein-based polymeric materials. The repeating units of the polypeptides consist of oligomeric alanylglycine sequences interspersed with glutamic acid residues inserted at intervals of 8 to 14 amino acids. Crystallization of such materials from formic acid produces β-sheet structures in the solid state, as shown by vibrational spectroscopy, nuclear magnetic resonance spectroscopy, and wide-angle x-ray diffraction. The diffraction results, together with observations from electron microscopy, are consistent with the formation of needle-shaped lamellar crystals whose thickness is controlled by the periodicity of the primary sequence. These results can be used to control solid-state structure in macromolecular materials.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1126/science.8073284DOIArticle
http://www.sciencemag.org/content/265/5177/1427PublisherArticle
http://www.jstor.org/stable/2884827JSTORArticle
ORCID:
AuthorORCID
Tirrell, David A.0000-0003-3175-4596
Alternate Title:Chemical sequence control of beta-sheet assembly in macromolecular crystals of periodic polypeptides
Additional Information:© 1994 American Association for the Advancement of Science. 4 April 1994; accepted 7 July 1994. We thank J. Cappello for the gift of p937.51 and for helpful advice. S. L. Hsu for guidance in vibrational spectroscopy and L. K. Thompson for help in obtaining solid-state NMR spectra. Y. Deguchi developed methods for the preparation of polymers 1b through 1d. We are grateful to A. D. Parkhe for preparing Fig. 2 and to S. Cooper for Fig. 8. Supported by grants from the Polymers and Genetics Programs of the National Science Foundation (NSF) and by the NSF Materials Research Laboratory at the University of Massachusetts.
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Funding AgencyGrant Number
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Issue or Number:5177
Record Number:CaltechAUTHORS:KREs1994
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:KREs1994
Official Citation:Chemical sequence control of beta-sheet assembly in macromolecular crystals of periodic polypeptides MT Krejchi, ED Atkins, AJ Waddon, MJ Fournier, TL Mason, and DA Tirrell Science 2 September 1994: 265 (5177), 1427-1432. [DOI:10.1126/science.8073284]
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53489
Collection:CaltechAUTHORS
Deposited By: Anne Hormann
Deposited On:27 Jan 2015 18:01
Last Modified:03 Oct 2019 07:50

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