CaltechAUTHORS
  A Caltech Library Service

Analysis of artificial proteins by matrix-assisted laser desorption mass spectrometry

Beavis, Ronald C. and Chait, Brian T. and Creel, Howard S. and Fournier, Maurille J. and Mason, Thomas L. and Tirrell, David A. (1992) Analysis of artificial proteins by matrix-assisted laser desorption mass spectrometry. Journal of the American Chemical Society, 114 (19). pp. 7584-7585. ISSN 0002-7863. https://resolver.caltech.edu/CaltechAUTHORS:BEAjacs1992

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:BEAjacs1992

Abstract

The growing use of recombinant DNA methods for the preparation of artificial proteins has created a pressing need for techniques for the rapid analysis of macromolecular structure. We report herein the use of matrix-assisted laser desorption mass spectrometry to determine the structure and purity of an artificial copolypeptide prepared as part of our ongoing investigation of the crystallization behavior of periodic protein. The mass spectra demonstrate that SDS polyacrylamide gel electrophoresis (SDS-PAGE) is insensitive to the presence of degraded fragments of the artificial protein and that molecular masses estimated by SDS-PAGE may be in error by more than 100%. Furthermore, accurate mass determination by matrix-assisted laser desorption mass spectrometry allowed the discovery of two previously undetected mutations in the DNA code for the protein.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja00045a053DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja00045a053PublisherArticle
ORCID:
AuthorORCID
Tirrell, David A.0000-0003-3175-4596
Additional Information:© 1992 American Chemical Society. Received April 13, 1992. This work was supported by grants from the National Science Foundation (DMR 8914359) and the National Institutes of Health (RR00862, GM38724, and BRSG S07 RR07065).
Funders:
Funding AgencyGrant Number
NSFDMR 8914359
NIHRR00862
NIHGM38724
NIHBRSG S07 RR07065
Issue or Number:19
Record Number:CaltechAUTHORS:BEAjacs1992
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:BEAjacs1992
Official Citation:Analysis of artificial proteins by matrix-assisted laser desorption mass spectrometry Ronald C. Beavis, Brian T. Chait, Howard S. Creel, Maurille J. Fournier, Thomas L. Mason, and David A. Tirrell Journal of the American Chemical Society 1992 114 (19), 7584-7585
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53498
Collection:CaltechAUTHORS
Deposited By: Anne Hormann
Deposited On:27 Jan 2015 00:11
Last Modified:03 Oct 2019 07:50

Repository Staff Only: item control page