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Mechanically Tunable Thin Films of Photosensitive Artificial Proteins:  Preparation and Characterization by Nanoindentation

Nowatzki, Paul J. and Franck, Christian and Maskarinec, Stacey A. and Ravichandran, Guruswami and Tirrell, David A. (2008) Mechanically Tunable Thin Films of Photosensitive Artificial Proteins:  Preparation and Characterization by Nanoindentation. Macromolecules, 41 (5). pp. 1839-1845. ISSN 0024-9297. http://resolver.caltech.edu/CaltechAUTHORS:20150112-113738681

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Abstract

Thin films of controlled elastic modulus were made by photo-cross-linking artificial extracellular matrix (aECM) proteins containing the photosensitive amino acid p-azidophenylalanine (pN_3Phe). The elastic moduli of the films were calculated from nanoindentation data collected by atomic force microscopy (AFM) using a thin-film Hertz model. The modulus was shown to be tunable in the range 0.3−1.0 MPa either by controlling the irradiation time or by varying the level of pN_3Phe in the protein. Tensile measurements on bulk films of the same proteins and finite-element simulation of the indentation process agreed with the thin-film modulus measurements from AFM. Substrates characterized by spatial variation in elastic modulus were created by local control of the irradiation time.


Item Type:Article
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http://dx.doi.org/10.1021/ma071717aDOIArticle
http://pubs.acs.org/doi/abs/10.1021/ma071717aPublisherArticle
Additional Information:© 2008 American Chemical Society. Received July 31, 2007. Revised Manuscript Received December 12, 2007. Published on Web 02/15/2008. Published In Issue March 11, 2008. We gratefully acknowledge support of this research by the Center for the Science and Engineering of Materials at Caltech (NSF DMR-0520565) and by NIH grant EB1971. S.A.M. is supported by a pre-doctoral fellowship from the NIH. We thank Marissa Mock for NMR characterization and Doron Shilo for help with AFM measurements.
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Funders:
Funding AgencyGrant Number
NSFDMR-0520565
NIHEB1971
NIH Predoctoral FellowshipGM007616
Record Number:CaltechAUTHORS:20150112-113738681
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20150112-113738681
Official Citation:Mechanically Tunable Thin Films of Photosensitive Artificial Proteins:  Preparation and Characterization by Nanoindentation. Paul J. Nowatzki, Christian Franck, Stacey A. Maskarinec, Guruswami Ravichandran, and David A. Tirrell. Macromolecules 2008 41 (5), 1839-1845.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53575
Collection:CaltechAUTHORS
Deposited By: Katherine Johnson
Deposited On:12 Jan 2015 19:41
Last Modified:29 Sep 2016 23:16

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