Nowatzki, Paul J. and Franck, Christian and Maskarinec, Stacey A. and Ravichandran, Guruswami and Tirrell, David A. (2008) Mechanically Tunable Thin Films of Photosensitive Artificial Proteins: Preparation and Characterization by Nanoindentation. Macromolecules, 41 (5). pp. 1839-1845. ISSN 0024-9297. doi:10.1021/ma071717a. https://resolver.caltech.edu/CaltechAUTHORS:20150112-113738681
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Abstract
Thin films of controlled elastic modulus were made by photo-cross-linking artificial extracellular matrix (aECM) proteins containing the photosensitive amino acid p-azidophenylalanine (pN_3Phe). The elastic moduli of the films were calculated from nanoindentation data collected by atomic force microscopy (AFM) using a thin-film Hertz model. The modulus was shown to be tunable in the range 0.3−1.0 MPa either by controlling the irradiation time or by varying the level of pN_3Phe in the protein. Tensile measurements on bulk films of the same proteins and finite-element simulation of the indentation process agreed with the thin-film modulus measurements from AFM. Substrates characterized by spatial variation in elastic modulus were created by local control of the irradiation time.
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| Additional Information: | © 2008 American Chemical Society. Received July 31, 2007. Revised Manuscript Received December 12, 2007. Published on Web 02/15/2008. Published In Issue March 11, 2008. We gratefully acknowledge support of this research by the Center for the Science and Engineering of Materials at Caltech (NSF DMR-0520565) and by NIH grant EB1971. S.A.M. is supported by a pre-doctoral fellowship from the NIH. We thank Marissa Mock for NMR characterization and Doron Shilo for help with AFM measurements. | |||||||||
| Group: | GALCIT | |||||||||
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| Issue or Number: | 5 | |||||||||
| DOI: | 10.1021/ma071717a | |||||||||
| Record Number: | CaltechAUTHORS:20150112-113738681 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20150112-113738681 | |||||||||
| Official Citation: | Mechanically Tunable Thin Films of Photosensitive Artificial Proteins: Preparation and Characterization by Nanoindentation. Paul J. Nowatzki, Christian Franck, Stacey A. Maskarinec, Guruswami Ravichandran, and David A. Tirrell. Macromolecules 2008 41 (5), 1839-1845. | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 53575 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Katherine Johnson | |||||||||
| Deposited On: | 12 Jan 2015 19:41 | |||||||||
| Last Modified: | 10 Nov 2021 20:04 |
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