Connor, Rebecca E. and Piatkov, Konstantin and Varshavsky, Alexander and Tirrell, David A. (2008) Enzymatic N-terminal Addition of Noncanonical Amino Acids to Peptides and Proteins. ChemBioChem, 9 (3). pp. 366-369. ISSN 1439-4227. doi:10.1002/cbic.200700605. https://resolver.caltech.edu/CaltechAUTHORS:20150112-114337627
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Abstract
A chromatographic assay was used to identify ten noncanonical amino acids that can be appended to the N terminus of peptides and proteins by the L,F-transferase of Escherichia coli. A model protein substrate, E. coli dihydrofolate reductase, was modified with p-ethynylphenylalanine and conjugated to azide–biotin and azide–polyethylene glycol–fluorescein probes (see scheme).
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| Additional Information: | © 2008 WILEY-VCH Verlag GmbH & Co. Received: October 10, 2007. Published online on January 18, 2008. This work was supported by NIH grant GM62523 (to D.A.T.), NIH grant DK39520 (to A.V.), the ARO-sponsored Institute for Collaborative Biotechnologies, and the Beckman Institute at Caltech. We thank Christina Smolke for use of the Molecular Imager and Mona Shahgholi for help with mass spectrometry. Pin Wang and A. James Link kindly provided the purified synthetases. Edman degradation was performed by the Peptide and Protein Molecular Analysis Laboratory of the Beckman Institute at Caltech. | ||||||||||||
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| Subject Keywords: | amino acids; peptides; proteins; transferases | ||||||||||||
| Issue or Number: | 3 | ||||||||||||
| DOI: | 10.1002/cbic.200700605 | ||||||||||||
| Record Number: | CaltechAUTHORS:20150112-114337627 | ||||||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20150112-114337627 | ||||||||||||
| Official Citation: | Connor, R. E., Piatkov, K., Varshavsky, A. and Tirrell, D. A. (2008), Enzymatic N-terminal Addition of Noncanonical Amino Acids to Peptides and Proteins. ChemBioChem, 9: 366–369. doi: 10.1002/cbic.200700605 | ||||||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||||
| ID Code: | 53576 | ||||||||||||
| Collection: | CaltechAUTHORS | ||||||||||||
| Deposited By: | Katherine Johnson | ||||||||||||
| Deposited On: | 12 Jan 2015 19:58 | ||||||||||||
| Last Modified: | 10 Nov 2021 20:04 |
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