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Visible-Light-Initiated Thiol−Acrylate Photopolymerization of Heparin-Based Hydrogels

Fu, Amy and Gwon, Kihak and Kim, Mihye and Tae, Giyoong and Kornfield, Julia A. (2015) Visible-Light-Initiated Thiol−Acrylate Photopolymerization of Heparin-Based Hydrogels. Biomacromolecules, 16 (2). pp. 497-506. ISSN 1525-7797. https://resolver.caltech.edu/CaltechAUTHORS:20150113-070659617

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Abstract

An in situ heparin-based forming hydrogel that cures under visible-light is formulated using eosin Y as a photoinitiator with triethanolamine as an electron donor to initiate reaction of thiolated-heparin with acrylate-ended poly(ethylene glycol). Formulations and irradiation conditions are presented for control of heparin content (1.6 to 3.3% w/v), modulus (100–10 000 Pa), and gelation time (30–600 s). Encapsulation of 3T3 fibroblasts in the hydrogel gave over 96% viability for all conditions examined. In vitro characterization of epidermal growth factor released from the hydrogel confirmed that the growth factor remains bioactive. The ability to deliver growth factors, fast gelation kinetics under visible light, and independent control of physical and biochemical properties makes this system a promising candidate for use in regenerative medicine. In particular, irradiation conditions that achieve gelation in 150s are compatible with the stringent light exposure limits of the retina, which affords a wide safety margin for use with other tissues.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/bm501543aDOIArticle
http://pubs.acs.org/doi/abs/10.1021/bm501543aPublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/bm501543aRelated ItemSupporting Information
ORCID:
AuthorORCID
Kornfield, Julia A.0000-0001-6746-8634
Additional Information:© 2014 American Chemical Society. Received: October 21, 2014. Revised: December 16, 2014. Publication Date (Web): December 23, 2014. This project was supported by the “GIST-Caltech Research Collaboration Project” through a grant provided by the Gwangju Institute of Science and Technology, Korea. We thank Dr. Matthew Mattson and Karthik Ramachandran for their assistance in using ZEMAX ray tracing software.
Funders:
Funding AgencyGrant Number
Gwangju Institute of Science and Technology, KoreaUNSPECIFIED
Issue or Number:2
Record Number:CaltechAUTHORS:20150113-070659617
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150113-070659617
Official Citation:Visible-Light-Initiated Thiol–Acrylate Photopolymerization of Heparin-Based Hydrogels Amy Fu, Kihak Gwon, Mihye Kim, Giyoong Tae, and Julia A. Kornfield Biomacromolecules 2015 16 (2), 497-506 DOI: 10.1021/bm501543a
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53601
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:13 Jan 2015 15:46
Last Modified:03 Oct 2019 07:51

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