Lee, Chongmok and Kwak, Juhyoun and Bard, Allen J. (1990) Application of scanning electrochemical microscopy to biological samples. Proceedings of the National Academy of Sciences of the United States of America, 87 (5). pp. 1740-1743. ISSN 0027-8424. PMCID PMC53558. doi:10.1073/pnas.87.5.1740. https://resolver.caltech.edu/CaltechAUTHORS:20150113-115051446
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Abstract
The scanning electrochemical microscope can be used in the feedback mode in two-dimensional scans over biological substrates to obtain topographic information at the micrometer level. In this mode, the effect of distance between a substrate (either conductive or insulating) and a scanning ultramicroelectrode tip on the electrolytic current flowing at the tip is recorded as a function of the tip x-y position. Scans of the upper surface of a grass leaf and the lower surface of a Ligustrum sinensis leaf (which show open stomata structures) immersed in aqueous solution are shown. Scans of the upper surface of an elodea leaf in the dark and under irradiation, where the tip reaction is the reduction of oxygen produced by photosynthesis, demonstrate the possibility of obtaining information about the distribution of reaction sites on the substrate surface.
| Item Type: | Article | |||||||||
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| Additional Information: | © 1990 National Academy of Sciences. Contributed by Allen J. Bard, December 18, 1989. The authors appreciate helpful discussions and advice by Dr. Ki Joong Kim. The support of this research by the Robert A. Welch Foundation and the National Science Foundation (CHE8901450) is gratefully acknowledged. | |||||||||
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| Issue or Number: | 5 | |||||||||
| PubMed Central ID: | PMC53558 | |||||||||
| DOI: | 10.1073/pnas.87.5.1740 | |||||||||
| Record Number: | CaltechAUTHORS:20150113-115051446 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20150113-115051446 | |||||||||
| Official Citation: | Lee, C., Kwak, J., & Bard, A. J. (1990). Application of scanning electrochemical microscopy to biological samples. Proceedings of the National Academy of Sciences, 87(5), 1740-1743. | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 53629 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Jason Perez | |||||||||
| Deposited On: | 13 Jan 2015 22:53 | |||||||||
| Last Modified: | 10 Nov 2021 20:04 |
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