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Dynamic and structural properties of polymerized phosphatidylcholine vesicle membranes

Kusumi, Akihiro and Singh, Maninder and Tirrell, David A. and Oehme, Gunther and Singh, Alok and Samuel, N. K. P. and Hyde, James S. and Regen, Steven L. (1983) Dynamic and structural properties of polymerized phosphatidylcholine vesicle membranes. Journal of the American Chemical Society, 105 (10). pp. 2975-2980. ISSN 0002-7863. https://resolver.caltech.edu/CaltechAUTHORS:KUSjacs1983

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Abstract

The phase transition, fluidity, and polarity properties of polymerized and nonpolymerized vesicle membranes derived from 1-palmitoyl-2-[12(methacryloyloxy)dodecanoyl]-L-α-phosphatidylcholine (1), bis[12-(methacryloyloxy)dodecanoyl] -L-α-phosphatidylcholine (2), and 1,2-dipalmitoyl[N-2-(methacryloyloxy)ethyl]-DL-α-phosphatidylcholine (3) have been examined by means of differential scanning calorimetry and by the spin-labeling technique. Above 5 °C aqueous multilamellar dispersions of 1, 2, and 3 show a broad-phase transition, no phase change, and a sharp transition, respectively. Polymerization of the methacrylate group causes a decrease in membrane fluidity in each case and an increase in the temperature of the gross phase change for 1 and 3. Membrane fluidity in copolymerized vesicles of 1 and 2 decreases linearly with the mole fraction of 2 used. The polarity gradient in membranes of 3 is small; polarity gradients in membranes of 1 and 2 are large. Polymerization has no noticeable effect on these gradients. Fluidity gradients are also present in membranes of 1, 2, and 3 above 20 °C. Polymerization alters the fluidity gradients in a manner which is comparable to lowering the temperature; it also reduces the ability of each membrane to bind [^(15)N]perdeuteriotempone. These results are compared directly with the dynamic and structural properties found in conventional liposomes derived from dipalmitoylphosphatidylcholine and egg yolk phosphatidylcholine.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja00348a009DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja00348a009PublisherArticle
ORCID:
AuthorORCID
Tirrell, David A.0000-0003-3175-4596
Additional Information:© 1983 American Chemical Society. Received September 7, 1982. This work was supported by PHS Grant No. CA 28891 awarded by the National Cancer Institute and Grants RR-01008 and GM-22923 from the National Institutes of Health. Registry No. 1, 79481-27-9; 2, 79605-84-8; 3, 85168-67-8; tempone, 2896-70-0.
Funders:
Funding AgencyGrant Number
Alfred P. Sloan FoundationUNSPECIFIED
NIHCA 28891
NIHGM-22923
National Cancer InstituteUNSPECIFIED
Issue or Number:10
Record Number:CaltechAUTHORS:KUSjacs1983
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:KUSjacs1983
Official Citation:Dynamic and structural properties of polymerized phosphatidylcholine vesicle membranes Akihiro Kusumi, Maninder Singh, David A. Tirrell, Gunther Oehme, Alok Singh, N. K. P. Samuel, James S. Hyde, and Steven L. Regen Journal of the American Chemical Society 1983 105 (10), 2975-2980
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53648
Collection:CaltechAUTHORS
Deposited By: Anne Hormann
Deposited On:16 Jan 2015 20:51
Last Modified:03 Oct 2019 07:51

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