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Biosynthesis of Proteins Incorporating a Versatile Set of Phenylalanine Analogues

Kirshenbaum, Kent and Carrico, Isaac S. and Tirrell, David A. (2002) Biosynthesis of Proteins Incorporating a Versatile Set of Phenylalanine Analogues. ChemBioChem, 3 (2-3). pp. 235-237. ISSN 1439-4227. https://resolver.caltech.edu/CaltechAUTHORS:20150114-094948843

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Abstract

Unnatural amino acids with useful chemical functionality can replace phenylalanine in bacterial proteins. Coexpression of a promiscuous phenylalanine-tRNA synthetase mutant enables the synthesis of target proteins bearing iodophenyl, cyanophenyl, ethynylphenyl, azidophenyl, and pyridyl groups (see general structures). Proteins incorporating the analogues have a range of potential applications, including Pd-mediated conjugation (R=CCH), photoaffinity labeling (R=N_3), X-ray phasing (R=I), and novel metal coordination (R=pyridyl).


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1002/1439-7633(20020301)3:2/3<235::AID-CBIC235>3.0.CO;2-7DOIArticle
http://onlinelibrary.wiley.com/doi/10.1002/1439-7633%2820020301%293:2/3%3C235::AID-CBIC235%3E3.0.CO;2-7/abstractUNSPECIFIEDArticle
ORCID:
AuthorORCID
Tirrell, David A.0000-0003-3175-4596
Additional Information:© 1999-2014 John Wiley & Sons, Inc. Issue published online: 7 MAR 2002. Article first published online: 7 MAR 2002. Manuscript Received: 10 OCT 2001. This work was supported by the National Institutes of Health through a grant (no.: RO1-GM62523-01), a postdoctoral fellowship to K.K. (no.: F32-GM29474), and a training grant stipend to I.S.C. (no.: T32-GM08501). We thank Nandita Sharma, Ian Suydam, Steven Boxer, Melanie Bennett, Mona Shahgholi, Hanna Rapaport, and Yi Tang for their advice and assistance.
Funders:
Funding AgencyGrant Number
NIHRO1-GM62523-01
NIH Postdoctoral FellowshipF32-GM29474
NIHT32-GM08501
Subject Keywords:amino acids; biosynthesis; mutagenesis; photoaffinity labeling; protein modifications
Issue or Number:2-3
Record Number:CaltechAUTHORS:20150114-094948843
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150114-094948843
Official Citation:Kirshenbaum, K., Carrico, I. S. and Tirrell, D. A. (2002), Biosynthesis of Proteins Incorporating a Versatile Set of Phenylalanine Analogues. ChemBioChem, 3: 235–237. doi: 10.1002/1439-7633(20020301)3:2/3<235::AID-CBIC235>3.0.CO;2-7
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53682
Collection:CaltechAUTHORS
Deposited By: Katherine Johnson
Deposited On:14 Jan 2015 17:59
Last Modified:03 Oct 2019 07:51

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