Kirshenbaum, Kent and Carrico, Isaac S. and Tirrell, David A. (2002) Biosynthesis of Proteins Incorporating a Versatile Set of Phenylalanine Analogues. ChemBioChem, 3 (2-3). pp. 235-237. ISSN 1439-4227. https://resolver.caltech.edu/CaltechAUTHORS:20150114-094948843
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Abstract
Unnatural amino acids with useful chemical functionality can replace phenylalanine in bacterial proteins. Coexpression of a promiscuous phenylalanine-tRNA synthetase mutant enables the synthesis of target proteins bearing iodophenyl, cyanophenyl, ethynylphenyl, azidophenyl, and pyridyl groups (see general structures). Proteins incorporating the analogues have a range of potential applications, including Pd-mediated conjugation (R=CCH), photoaffinity labeling (R=N_3), X-ray phasing (R=I), and novel metal coordination (R=pyridyl).
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| Additional Information: | © 1999-2014 John Wiley & Sons, Inc. Issue published online: 7 MAR 2002. Article first published online: 7 MAR 2002. Manuscript Received: 10 OCT 2001. This work was supported by the National Institutes of Health through a grant (no.: RO1-GM62523-01), a postdoctoral fellowship to K.K. (no.: F32-GM29474), and a training grant stipend to I.S.C. (no.: T32-GM08501). We thank Nandita Sharma, Ian Suydam, Steven Boxer, Melanie Bennett, Mona Shahgholi, Hanna Rapaport, and Yi Tang for their advice and assistance. | |||||||||
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| Subject Keywords: | amino acids; biosynthesis; mutagenesis; photoaffinity labeling; protein modifications | |||||||||
| Issue or Number: | 2-3 | |||||||||
| Record Number: | CaltechAUTHORS:20150114-094948843 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20150114-094948843 | |||||||||
| Official Citation: | Kirshenbaum, K., Carrico, I. S. and Tirrell, D. A. (2002), Biosynthesis of Proteins Incorporating a Versatile Set of Phenylalanine Analogues. ChemBioChem, 3: 235–237. doi: 10.1002/1439-7633(20020301)3:2/3<235::AID-CBIC235>3.0.CO;2-7 | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 53682 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Katherine Johnson | |||||||||
| Deposited On: | 14 Jan 2015 17:59 | |||||||||
| Last Modified: | 03 Oct 2019 07:51 |
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