Lithographic Patterning of Photoreactive Cell-Adhesive Proteins

Carrico, Isaac S. and Maskarinec, Stacey A. and Heilshorn, Sarah C. and Mock, Marissa L. and Liu, Julie C. and Nowatzki, Paul J. and Franck, Christian and Ravichandran, Guruswami and Tirrell, David A. (2007) Lithographic Patterning of Photoreactive Cell-Adhesive Proteins. Journal of the American Chemical Society, 129 (16). pp. 4874-4785. ISSN 0002-7863. PMCID PMC2533112. doi:10.1021/ja070200b. https://resolver.caltech.edu/CaltechAUTHORS:20150114-104001187

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Abstract

We describe a novel, simple method for the photolithographic patterning of cell-adhesive proteins. Intrinsically photoreactive proteins are synthesized in Escherichia coli through incorporation of the non-canonical, photosensitive amino acid para-azidophenylalanine. Upon ultraviolet irradiation at 365 nm, proteins form cross-linked films with elastic moduli that can be tuned by varying the concentration of photoreactive amino acid in the expression medium. Films of these proteins can be directly patterned using standard photolithographic techniques. We demonstrate the utility of this method of protein patterning by creating stable arrays of fibroblast cells on an engineered protein “photoresist”.

Item Type:

Article

Related URLs:

URL	URL Type	Description
http://dx.doi.org/10.1021/ja070200b	DOI	Article
http://pubs.acs.org/doi/abs/10.1021/ja070200b	Publisher	Article
http://pubs.acs.org/doi/suppl/10.1021/ja070200b	Publisher	Supplemental material
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2533112/	PubMed Central	Article

ORCID:

Author	ORCID
Ravichandran, Guruswami	0000-0002-2912-0001
Tirrell, David A.	0000-0003-3175-4596

Additional Information:

Copyright © 2007 American Chemical Society. Received January 10, 2007 . Publication Date (Web): March 31, 2007. Published In Issue April 25, 2007. We thank Michael Diehl, Alireza Ghaffari, and Nandita Sharma for helpful discussion, and Kechun Zhang for help in preparing the patterned substrates. Supported by the NSF Center for the Science and Engineering of Materials at Caltech, NIH GM62523 and EB01971, an NIH predoctoral fellowship to S.A.M., a Whitaker Graduate Fellowship to J.C.L., and the Joseph J. Jacobs Institute for Molecular Engineering for Medicine.

Group:

GALCIT

Funders:

Funding Agency	Grant Number
NSF	UNSPECIFIED
NIH	GM62523
NIH	EB01971
NIH Predoctoral Fellowship	UNSPECIFIED
Whitaker Graduate Fellowship	UNSPECIFIED
Joseph J. Jacobs Institute for Molecular Engineering for Medicine	UNSPECIFIED

Issue or Number:

PubMed Central ID:

PMC2533112

DOI:

10.1021/ja070200b

Record Number:

CaltechAUTHORS:20150114-104001187

Persistent URL:

https://resolver.caltech.edu/CaltechAUTHORS:20150114-104001187

Official Citation:

Lithographic Patterning of Photoreactive Cell-Adhesive Proteins Isaac S. Carrico, Stacey A. Maskarinec, Sarah C. Heilshorn, Marissa L. Mock, Julie C. Liu, Paul J. Nowatzki, Christian Franck, Guruswami Ravichandran, and David A. Tirrell Journal of the American Chemical Society 2007 129 (16), 4874-4875

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ID Code:

53698

Collection:

CaltechAUTHORS

Deposited By:

Katherine Johnson

Deposited On:

14 Jan 2015 19:13

Last Modified:

10 Nov 2021 20:05

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