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Artificial Polypeptide Scaffold for Protein Immobilization

Zhang, Kechun and Diehl, Michael R. and Tirrell, David A. (2005) Artificial Polypeptide Scaffold for Protein Immobilization. Journal of the American Chemical Society, 127 (29). pp. 10136-10137. ISSN 0002-7863. https://resolver.caltech.edu/CaltechAUTHORS:20150114-104001317

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Abstract

An artificial polypeptide scaffold composed of surface anchor and protein capture domains was designed and expressed in vivo. By using a mutant E. coli phenylalanyl−tRNA synthetase, the photoreactive amino acid para-azidophenylalanine was incorporated into the surface anchor domain. Octyltrichlorosilane-treated surfaces were functionalized with this polypeptide by spin coating and photocrosslinking. The resulting protein films were shown to immobilize recombinant proteins through association of coiled coil heterodimer.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja051457hDOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja051457hPublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/ja051457hPublisherSupplemental material
ORCID:
AuthorORCID
Tirrell, David A.0000-0003-3175-4596
Additional Information:Copyright © 2005 American Chemical Society. Published In Issue July 27, 2005. Publication Date (Web): June 29, 2005. Received March 7, 2005. This work was supported by the NSF sponsored Center for the Science and Engineering of Materials at the California Institute of Technology.
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Issue or Number:29
Record Number:CaltechAUTHORS:20150114-104001317
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150114-104001317
Official Citation:Artificial Polypeptide Scaffold for Protein Immobilization Kechun Zhang,Michael R. Diehl, and, and David A. Tirrell* Journal of the American Chemical Society 2005 127 (29), 10136-10137
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53700
Collection:CaltechAUTHORS
Deposited By: Katherine Johnson
Deposited On:14 Jan 2015 20:02
Last Modified:03 Oct 2019 07:51

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