Zhang, Kechun and Diehl, Michael R. and Tirrell, David A. (2005) Artificial Polypeptide Scaffold for Protein Immobilization. Journal of the American Chemical Society, 127 (29). pp. 10136-10137. ISSN 0002-7863. https://resolver.caltech.edu/CaltechAUTHORS:20150114-104001317
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Abstract
An artificial polypeptide scaffold composed of surface anchor and protein capture domains was designed and expressed in vivo. By using a mutant E. coli phenylalanyl−tRNA synthetase, the photoreactive amino acid para-azidophenylalanine was incorporated into the surface anchor domain. Octyltrichlorosilane-treated surfaces were functionalized with this polypeptide by spin coating and photocrosslinking. The resulting protein films were shown to immobilize recombinant proteins through association of coiled coil heterodimer.
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| Additional Information: | Copyright © 2005 American Chemical Society. Published In Issue July 27, 2005. Publication Date (Web): June 29, 2005. Received March 7, 2005. This work was supported by the NSF sponsored Center for the Science and Engineering of Materials at the California Institute of Technology. | ||||||||||||
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| Issue or Number: | 29 | ||||||||||||
| Record Number: | CaltechAUTHORS:20150114-104001317 | ||||||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20150114-104001317 | ||||||||||||
| Official Citation: | Artificial Polypeptide Scaffold for Protein Immobilization Kechun Zhang,Michael R. Diehl, and, and David A. Tirrell* Journal of the American Chemical Society 2005 127 (29), 10136-10137 | ||||||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||||
| ID Code: | 53700 | ||||||||||||
| Collection: | CaltechAUTHORS | ||||||||||||
| Deposited By: | Katherine Johnson | ||||||||||||
| Deposited On: | 14 Jan 2015 20:02 | ||||||||||||
| Last Modified: | 03 Oct 2019 07:51 |
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