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Second Structural Motif for Recognition of DNA by Oligonucleotide-Directed Triple-Helix Formation

Beal, Peter A. and Dervan, Peter B. (1991) Second Structural Motif for Recognition of DNA by Oligonucleotide-Directed Triple-Helix Formation. Science, 251 (4999). pp. 1360-1363. ISSN 0036-8075. doi:10.1126/science.2003222.

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Relative orientations of the DNA strands within a purine.purine.pyrimidine triple helix have been determined by affinity cleaving. A purine-rich oligonucleotide bound in the major groove of double-helical DNA antiparallel to the Watson-Crick purine strand. Binding depended upon the concentration of multivalent cations such as spermine or Mg^(2+), and appeared to be relatively independent of pH. Two models with specific hydrogen-bonding patterns for base triplets (G.GC, A.AT, and T.AT) are proposed to explain the sequence specificity of binding. The two models differ in the conformation about the glycosyl bond (syn or anti) and the location of the phosphate-deoxyribose backbone in the major groove of DNA. This motif broadens the structural frameworks available as a basis for the design of sequence-specific DNA binding molecules.

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Dervan, Peter B.0000-0001-8852-7306
Additional Information:© 1991 American Association for the Advancement of Science. Received 22 August 1990; accepted 4 January 1991. We are grateful to NIH for generous grant support and to DOE for a predoctoral fellowship to P.A.B.
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Department of Energy (DOE)UNSPECIFIED
Issue or Number:4999
Record Number:CaltechAUTHORS:20150114-104814879
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Official Citation:Second structural motif for recognition of DNA by oligonucleotide-directed triple-helix formation PA Beal and PB Dervan Science 15 March 1991: 251 (4999), 1360-1363. [DOI:10.1126/science.2003222]
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53702
Deposited By: Tony Diaz
Deposited On:14 Jan 2015 20:41
Last Modified:10 Nov 2021 20:05

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