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Comparative Cell Response to Artificial Extracellular Matrix Proteins Containing the RGD and CS5 Cell-Binding Domains

Liu, Julie C. and Heilshorn, Sarah C. and Tirrell, David A. (2004) Comparative Cell Response to Artificial Extracellular Matrix Proteins Containing the RGD and CS5 Cell-Binding Domains. Biomacromolecules, 5 (2). pp. 497-504. ISSN 1525-7797. doi:10.1021/bm034340z. https://resolver.caltech.edu/CaltechAUTHORS:20150114-121449681

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Abstract

This study addresses endothelial cell adhesion and spreading on a family of artificial extracellular matrix (aECM) proteins designed for application in small-diameter vascular grafts. The aECM proteins contain domains derived from elastin and from fibronectin. aECM 1 contains the RGD sequence from the tenth type III domain of fibronectin; aECM 3 contains the fibronectin CS5 cell-binding domain. Negative control proteins aECM 2 and 4 are scrambled versions of aECM 1 and 3, respectively. Competitive peptide inhibition studies and comparisons of positive and negative control proteins confirm that adhesion of HUVECs to aECM proteins 1 and 3 is sequence specific. When subjected to a normal detachment force of 780 pN, 3-fold more HUVECs remained adherent to aECM 1 than to aECM 3. HUVECs also spread more rapidly on aECM 1 than on aECM 3. These results (i) indicate that cellular responses to aECM proteins can be modulated through choice of cell-binding domain and (ii) recommend the RGD sequence for applications that require rapid endothelial cell spreading and matrix adhesion.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/bm034340zDOIArticle
http://pubs.acs.org/doi/abs/10.1021/bm034340zPublisherArticle
ORCID:
AuthorORCID
Tirrell, David A.0000-0003-3175-4596
Additional Information:Copyright © 2004 American Chemical Society. Published In Issue March 08, 2004. Publication Date (Web): January 24, 2004. Received September 4, 2003. Revised Manuscript Received December 8, 2003. Acknowledgment. We thank Kathleen Di Zio for helpful discussions regarding the DNA cloning and protein purification, Anand Asthagiri for helpful discussions regarding the cell spreading studies, and Scott Fraser and Carole Lu for help with the fluorescence microscopy. This work was supported by NIH Grant 5 RO1 HL59987-03, NSF Grant BES-9901648, and a Whitaker graduate fellowship.
Funders:
Funding AgencyGrant Number
NIH5 RO1 HL59987-03
NSFBES-9901648
Whitaker Graduate FellowshipUNSPECIFIED
Issue or Number:2
DOI:10.1021/bm034340z
Record Number:CaltechAUTHORS:20150114-121449681
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150114-121449681
Official Citation: Comparative Cell Response to Artificial Extracellular Matrix Proteins Containing the RGD and CS5 Cell-Binding Domains Julie C. Liu,Sarah C. Heilshorn, and, and David A. Tirrell* Biomacromolecules 2004 5 (2), 497-504
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53713
Collection:CaltechAUTHORS
Deposited By: Katherine Johnson
Deposited On:15 Jan 2015 18:58
Last Modified:10 Nov 2021 20:06

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