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Effects of Temperature and Pressure on the Aggregation Properties of an Engineered Elastin Model Polypeptide in Aqueous Solution

Tamura, T. and Yamaoka, T. and Kunugi, S. and Panitch, A. and Tirrell, D. A. (2000) Effects of Temperature and Pressure on the Aggregation Properties of an Engineered Elastin Model Polypeptide in Aqueous Solution. Biomacromolecules, 1 (4). pp. 552-555. ISSN 1525-7797. http://resolver.caltech.edu/CaltechAUTHORS:20150114-121449864

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Abstract

The pressure and temperature dependence of the cloud point transition of an aqueous solution of an elastin-like polypeptide (MGLDGSMG(VPGIG)_(40)VPLE), prepared by bacterial expression of the corresponding artificial gene, was measured. A temperature−pressure diagram was constructed over a wide range of conditions. The (VPGIG)_(40) solution exhibited a well-defined pressure-induced cloudpoint (P_c), as well as a temperature-induced transition (T_c). From near atmospheric pressure up to 100 MPa, T_c increased with increasing pressure, but decreased with further increases in pressure above 200 MPa. The maximum T_c was reached at 100−200 MPa. Between 10 and 25 °C, the Pc decreased with increasing temperature, and a broad maximum in P_c was observed in the range −10 to 0 °C. These results are compared with our previous results on synthetic thermoresponsive vinyl polymers.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/bm005606uDOIArticle
http://pubs.acs.org/doi/abs/10.1021/bm005606uPublisherArticle
ORCID:
AuthorORCID
Tirrell, D. A.0000-0003-3175-4596
Additional Information:© 2000 American Chemical Society. Published In Issue December 12, 2000. Publication Date (Web): November 1, 2000. Received September 5, 2000. Revised Manuscript Received October 10, 2000.
Record Number:CaltechAUTHORS:20150114-121449864
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20150114-121449864
Official Citation:Effects of Temperature and Pressure on the Aggregation Properties of an Engineered Elastin Model Polypeptide in Aqueous Solution T. Tamura, T. Yamaoka, S. Kunugi, A. Panitch, and D. A. Tirrell Biomacromolecules 2000 1 (4), 552-555
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53715
Collection:CaltechAUTHORS
Deposited By: Katherine Johnson
Deposited On:15 Jan 2015 18:43
Last Modified:09 Nov 2016 22:33

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