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Engineering the Extracellular Matrix:  A Novel Approach to Polymeric Biomaterials. I. Control of the Physical Properties of Artificial Protein Matrices Designed to Support Adhesion of Vascular Endothelial Cells

Welsh, Eric R. and Tirrell, David A. (2000) Engineering the Extracellular Matrix:  A Novel Approach to Polymeric Biomaterials. I. Control of the Physical Properties of Artificial Protein Matrices Designed to Support Adhesion of Vascular Endothelial Cells. Biomacromolecules, 1 (1). pp. 23-30. ISSN 1525-7797. http://resolver.caltech.edu/CaltechAUTHORS:20150114-121450041

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Abstract

Methods of genetic engineering were applied to the design and biosynthesis of three extracellular matrix protein analogues constructed from identical elastin- and fibronectin-derived repeating units but characterized by different molecular weights in the range of 14 000 to 59 000. Expression levels were enhanced by the serendipitous choice of an N-terminal fusion sequence such that gram-scale syntheses were achieved for each protein. Purification protocols were developed that resulted in proteins of high purity and correct sequence, as determined by amino acid analysis, NMR spectroscopy, and lower critical solution temperature (LCST). Glutaraldehyde was shown to insolubilize the otherwise soluble proteins in a concentration-dependent manner. Tensile moduli of cross-linked protein films were measured and found to be inversely related to the molecular weights of the engineered proteins, which in each case corresponds to the theoretical molecular weight between cross-links. At the highest cross-link density (lowest molecular weight) the elastic modulus was similar to that of native elastin.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/bm0002914DOIArticle
http://pubs.acs.org/doi/abs/10.1021/bm0002914PublisherArticle
ORCID:
AuthorORCID
Tirrell, David A.0000-0003-3175-4596
Additional Information:Copyright © 2000 American Chemical Society. Published In Issue March 14, 2000. Publication Date (Web): February 4, 2000. Received January 17, 2000. Acknowledgment. This work was supported by National Institutes of Health grant HL59987.
Funders:
Funding AgencyGrant Number
NIHHL59987
Record Number:CaltechAUTHORS:20150114-121450041
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20150114-121450041
Official Citation:Engineering the Extracellular Matrix:  A Novel Approach to Polymeric Biomaterials. I. Control of the Physical Properties of Artificial Protein Matrices Designed to Support Adhesion of Vascular Endothelial Cells Eric R. Welsh and David A. Tirrell Biomacromolecules 2000 1 (1), 23-30
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53717
Collection:CaltechAUTHORS
Deposited By: Katherine Johnson
Deposited On:15 Jan 2015 19:12
Last Modified:15 Jan 2015 20:06

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