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A pH Dependent Coil-to-Sheet Transition in a Periodic Artificial Protein Adsorbed at the Air-Water Interface

Chen, Huijuan and Hsu, Shaw Ling and Tirrell, David A. and Stidham, Howard D. (1997) A pH Dependent Coil-to-Sheet Transition in a Periodic Artificial Protein Adsorbed at the Air-Water Interface. Langmuir, 13 (18). pp. 4775-4778. ISSN 0743-7463. https://resolver.caltech.edu/CaltechAUTHORS:20150114-163429555

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Abstract

External infrared reflectance spectroscopy allows in situ determination of the conformational state of a periodic artificial protein adsorbed at the air−water interface. The protein, which consists of 36 copies of an octapeptide sequence [(AG)_3EG] constituted from alanine (A), glycine (G), and glutamic acid (E), adopts a random coil conformation in aqueous solution over a broad range of pH (5 < pH < 14). In contrast, the adsorbed chain undergoes a transition from a disordered (coil-like) state to a β-sheet conformation as the pH of the subphase is reduced. The conformational transition is offset by ca. 4 pH units from the titration curve of the polymer in aqueous solution, suggesting that the ionization behavior of the polymer is substantially perturbed by interfacial effects. In situ determination of the conformation of the adsorbed chain allows observation of a coil-to-sheet transition that is not observed in bulk solution.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/la970207eDOIArticle
http://pubs.acs.org/doi/abs/10.1021/la970207ePublisherArticle
ORCID:
AuthorORCID
Tirrell, David A.0000-0003-3175-4596
Additional Information:Copyright © 1997 American Chemical Society. Received February 25, 1997; Revised June 26, 1997. This work was supported by a grant from the Polymers and Genetics Program of the National Science Foundation (NSF) and by the NSF Materials Research Science and Engineering Center at the University of Massachusetts.
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NSFUNSPECIFIED
Issue or Number:18
Record Number:CaltechAUTHORS:20150114-163429555
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150114-163429555
Official Citation:A pH Dependent Coil-to-Sheet Transition in a Periodic Artificial Protein Adsorbed at the Air−Water Interface Huijuan Chen, Shaw Ling Hsu, and David A. Tirrell, and Howard D. Stidham Langmuir 1997 13 (18), 4775-4778
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53744
Collection:CaltechAUTHORS
Deposited By: Anne Hormann
Deposited On:15 Jan 2015 02:37
Last Modified:03 Oct 2019 07:52

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