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Synthetic Analogues of Membrane Proteins

Maeda, Mizuo and Kumano, Atsushi and Tirrell, David A. (1989) Synthetic Analogues of Membrane Proteins. In: Frontiers of Macromolecular Science. Blackwell Scientific , pp. 213-217. ISBN 0-632-02417-8 . http://resolver.caltech.edu/CaltechAUTHORS:20150114-164508803

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Abstract

Biological membranes carry a multitude of bound proteins and glycoproteins. These membrane-bound macromolecules confer on the membrane a variety of useful properties, including the capacity to respond to physical and chemical signals through changes in macromolecular conformation and solvation. We describe herein the preparation of synthetic or "semisynthetic" membranes that share with their natural counterparts two important characteristics: i). They are composed of a mixture of bilayer-forming lipids and bound macromolecules, and ii). They respond to well-defined chemical and physical signals via changes in macromolecular conformation and solvation. Poly(2-ethylacrylic acid) (PEAA) can be immobilized on phosphatidylcholine bilayer membranes by reaction of a thiolated derivative of the polymer with a membrane that includes a small amount of dimyristoyl N-[(4-maleimidomethyl)cyclohexanecarbonyl]phosphatidylethanolne. Bound and free polymer are readily separated by column chromatography. Typical preparations result in the irreversible binding of ca. 50 pg of PEAA per mg of lipid. Membranes prepared in this way are sensitive to hydrogen ion concentration, and are subject to large variations in permeability with small changes in pH. Rapid and quantitative release of vesicle contents can be achieved by mild acidification within the physiological pH range.


Item Type:Book Section
ORCID:
AuthorORCID
Tirrell, David A.0000-0003-3175-4596
Additional Information:© 1989 Blackwell Scientific. This work was supported by a Presidential Young Investigator Award of the National Science Foundation and by matching grants from the Exxon Education Foundation, the General Electric Company, and Air Products and Chemicals Co. We are grateful for leaves of absence granted by the University of Tokyo (to M. Maeda) and by the Japan Synthetic Rubber Co. (to A. Kumano).
Funders:
Funding AgencyGrant Number
NSFUNSPECIFIED
Exxon Education Foundation UNSPECIFIED
General ElectricUNSPECIFIED
Air Products and Chemicals Inc.UNSPECIFIED
Subject Keywords:Phospholipid; bilayer; polyelectrolyte; membrane proteins; controlled release
Record Number:CaltechAUTHORS:20150114-164508803
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20150114-164508803
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53746
Collection:CaltechAUTHORS
Deposited By: Anne Hormann
Deposited On:15 Jan 2015 02:23
Last Modified:15 Jan 2015 02:23

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