Chen, Jeannie and Makino, Clint L. and Peachey, Neal S. and Baylor, Denis A. and Simon, Melvin I. (1995) Mechanisms of rhodopsin inactivation in vivo as revealed by a COOH-terminal truncation mutant. Science, 267 (5196). pp. 374-377. ISSN 0036-8075. doi:10.1126/science.7824934. https://resolver.caltech.edu/CaltechAUTHORS:20150115-124616145
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Abstract
Although biochemical experiments suggest that rhodopsin and other receptors coupled to heterotrimeric guanosine triphosphate-binding proteins (G proteins) are inactivated by phosphorylation near the carboxyl (COOH)-terminus and the subsequent binding of a capping protein, little is known about the quenching process in vivo. Flash responses were recorded from rods of transgenic mice in which a fraction of the rhodopsin molecules lacked the COOH-terminal phosphorylation sites. In the single photon regime, abnormally prolonged responses, attributed to activation of individual truncated rhodopsins, occurred interspersed with normal responses. The occurrence of the prolonged responses suggests that phosphorylation is required for normal shutoff. Comparison of normal and prolonged single photon responses indicated that rhodopsin begins to be quenched before the peak of the electrical response and that quenching limits the response amplitude.
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Additional Information: | © 1995 American Association for the Advancement of Science. 21 June 1994; Accepted 10 November 1994. We thank T. Nakayama for making the transgene construct and B. Kobilka for his critique of an earlier version of the manuscript. The care and treatment of animals used in this study conformed to protocols approved by the respective institutions. Supported by the Ruth and Milton Steinbach Fund, the Department of Veteran's Affairs, the National Eye Institute (grants EY0570 and F32 EY06405), and the National Institute of Aging (NIH grant AG 12288). | ||||||||||||||
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Issue or Number: | 5196 | ||||||||||||||
DOI: | 10.1126/science.7824934 | ||||||||||||||
Record Number: | CaltechAUTHORS:20150115-124616145 | ||||||||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20150115-124616145 | ||||||||||||||
Official Citation: | Chen, J., Makino, C., Peachey, N., Baylor, D., & Simon, M. (1995). Mechanisms of rhodopsin inactivation in vivo as revealed by a COOH-terminal truncation mutant. Science, 267(5196), 374-377. doi: 10.1126/science.7824934 | ||||||||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||||||
ID Code: | 53787 | ||||||||||||||
Collection: | CaltechAUTHORS | ||||||||||||||
Deposited By: | INVALID USER | ||||||||||||||
Deposited On: | 16 Jan 2015 00:59 | ||||||||||||||
Last Modified: | 10 Nov 2021 20:07 |
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