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Characterization of early cortical population response to thalamocortical input in vitro

Hill, Michael R. H. and Greenfield, Susan A. (2014) Characterization of early cortical population response to thalamocortical input in vitro. Frontiers in Neuroscience, 7 . Art. No. 273. ISSN 1662-4548. PMCID PMC3907706. https://resolver.caltech.edu/CaltechAUTHORS:20150116-082003416

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Abstract

The in vitro thalamocortical slice preparation of mouse barrel cortex allows for stimulation of the cortex through its natural afferent thalamocortical pathway. This preparation was used here to investigate the first stage of cortical processing in the large postsynaptic dendritic networks as revealed by voltage sensitive dye imaging (VSDI). We identified the precise location and dimensions of two clearly distinguishable dendritic networks, one in the granular layer (GL) IV and one in the infragranular layer (IGL) V and VI and showed that they have different physiological properties. DiI fluorescent staining further revealed that thalamocortical axons project on to these two networks in the typical barrel like form, not only in the granular but also in the IGL. Finally we investigated the short-term dynamics of both the VSDI signal and the local field potential (LFP) in response to a train of eight-pulses at various frequencies in both these layers. We found evidence of differences in the plasticity between the first two response peaks compared to the remaining six peaks as well as differences in short-term plasticity between the VSDI response and the LFP. Our findings suggest, that at least early cortical processing takes place in two separate dendritic networks that may stand at the beginning of further parallel computation. The detailed characterization of the parameters of these networks may provide tools for further research into the complex dynamics of large dendritic networks and their role in cortical computation.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.3389/fnins.2013.00273 DOIArticle
http://journal.frontiersin.org/Journal/10.3389/fnins.2013.00273/fullPublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3907706/PubMed CentralArticle
Additional Information:© 2014 Hill and Greenfield. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Received: 04 October 2013; Paper pending published: 09 November 2013; Accepted: 23 December 2013; Published online: 31 January 2014. The research was financially supported by the Swiss Life Foundation, the Swiss Study Foundation and the Daniel Falkner Research Grant. Conflict of Interest Statement: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Funders:
Funding AgencyGrant Number
Swiss Life FoundationUNSPECIFIED
Swiss Study FoundationUNSPECIFIED
Daniel Falkner Research GrantUNSPECIFIED
Subject Keywords:thalamocortical, in vitro, mouse, barrel cortex, voltage sensitive dye, local field potential
PubMed Central ID:PMC3907706
Record Number:CaltechAUTHORS:20150116-082003416
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150116-082003416
Official Citation:Hill MRH and Greenfield SA (2014) Characterization of early cortical population response to thalamocortical input in vitro. Front. Neurosci. 7:273. doi: 10.3389/fnins.2013.00273
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53807
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:16 Jan 2015 18:05
Last Modified:03 Oct 2019 07:52

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