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Breaking the degeneracy of the genetic code

Kwon, Inchan and Kirshenbaum, Kent and Tirrell, David A. (2003) Breaking the degeneracy of the genetic code. Journal of the American Chemical Society, 125 (25). pp. 7512-7513. ISSN 0002-7863. https://resolver.caltech.edu/CaltechAUTHORS:20150116-102631008

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Abstract

A mutant yeast phenylalanine transfer RNA (ytRNA^(Phe)_(AAA)) containing a modified (AAA) anticodon was generated to explore the feasibility of breaking the degeneracy of the genetic code in Escherichia coli. By using an E. coli strain co-transformed with ytRNA^(Phe)_(AAA) and a mutant yeast phenylalanyl-tRNA synthetase, we demonstrate efficient replacement of phenylalanine (Phe) by L-3-(2-naphthyl)alanine (Nal) at UUU, but not at UUC codons.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja0350076 DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja0350076PublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/ja0350076PublisherSupplemental material
ORCID:
AuthorORCID
Tirrell, David A.0000-0003-3175-4596
Additional Information:Copyright © 2003 American Chemical Society. Published In Issue June 25, 2003. Publication Date (Web): May 29, 2003. Received March 5, 2003. Acknowledgment. This work was supported by NIH GM62523 and by the NSF MRSEC program. We thank Dr. Mona Shahgholi, Dr. Gary M. Hathaway, and Dr. Jie Zhou for mass spectrometry studies. We thank Pin Wang and Soojin Son for helpful discussions. Supporting Information Available: Protein expression gels and LC/MS/MS spectra (PDF). Also found in: Conference: 225th National Meeting of the American-Chemical-Society Location: New Orleans, LA. Date: MAR 23-27, 2003. Sponsor(s): Amer Chem Soc. Abstracts of Papers of the American Chemical Society. Volume: 225. Pages: U322-U322. Part: 2. Meeting Abstract: 255-ORGN. Published: MAR 2003.
Funders:
Funding AgencyGrant Number
NIHGM62523
NSF MRSECUNSPECIFIED
Issue or Number:25
Record Number:CaltechAUTHORS:20150116-102631008
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20150116-102631008
Official Citation: Breaking the Degeneracy of the Genetic Code Inchan Kwon,Kent Kirshenbaum, and, and David A. Tirrell* Journal of the American Chemical Society 2003 125 (25), 7512-7513
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53818
Collection:CaltechAUTHORS
Deposited By: Katherine Johnson
Deposited On:16 Jan 2015 19:13
Last Modified:03 Oct 2019 07:52

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