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Inhibition of DNA binding proteins by oligonucleotide-directed triple helix formation

Maher, Louis J., III and Wold, Barbara and Dervan, Peter B. (1989) Inhibition of DNA binding proteins by oligonucleotide-directed triple helix formation. Science, 245 (4919). pp. 725-730. ISSN 0036-8075. http://resolver.caltech.edu/CaltechAUTHORS:20150116-103837502

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Abstract

Oligonucleotides that bind to duplex DNA in a sequence-specific manner by triple helix formation offer an approach to the experimental manipulation of sequence-specific protein binding. Micromolar concentrations of pyrimidine oligodeoxyribonucleotides are shown to block recognition of double helical DNA by prokaryotic modifying enzymes and a eukaryotic transcription factor at a homopurine target site. Inhibition is sequence-specific. Oligonucleotides containing 5-methylcytosine provide substantially more efficient inhibition than oligonucleotides containing cytosine. The results have implications for gene-specific repression by oligonucleotides or their analogs.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1126/science.2549631DOIArticle
http://www.sciencemag.org/content/245/4919/725PublisherArticle
http://www.jstor.org/stable/1704035JSTORArticle
Additional Information:© 1989 American Association for the Advancement of Science. 21 March 1989; Accepted 5 July 1989. We thank R. Tjian for pSpl-516C, and we also thank members of the Dervan and Wold groups for helpful discussions. Supported by grants from the Caltech Beckman Institute and the NIH (B.W. and P.B.D.), by a Gosney Fellowship, and an American Cancer Society Fellowship to L.J.M.
Funders:
Funding AgencyGrant Number
Caltech Beckman Institute UNSPECIFIED
NIHUNSPECIFIED
Gosney FellowshipUNSPECIFIED
American Cancer SocietyUNSPECIFIED
Record Number:CaltechAUTHORS:20150116-103837502
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20150116-103837502
Official Citation:Maher, L., Wold, B., & Dervan, P. (1989). Inhibition of DNA binding proteins by oligonucleotide-directed triple helix formation. Science, 245(4919), 725-730. doi: 10.1126/science.2549631
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53822
Collection:CaltechAUTHORS
Deposited By: Joanne McCole
Deposited On:16 Jan 2015 19:49
Last Modified:16 Jan 2015 19:49

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