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Germline transmission of exogenous genes in the chicken

Bosselman, Robert A. and Hsu, Rou-Yin and Boggs, Tina and Hu, Sylvia and Bruszewski, Joan and Ou, Susan and Kozar, Lee and Martin, Frank and Green, Cal and Jacobsen, Frederick and Nicolson, Margery O. and Schultz, Joseph A. and Semon, Kenneth M. and Rishell, William and Stewart, R. Gregory (1989) Germline transmission of exogenous genes in the chicken. Science, 243 (4890). pp. 533-535. ISSN 0036-8075. doi:10.1126/science.2536194.

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Difficulties associated with in vitro manipulation and culture of the early chicken embryo have restricted generation of transgenic chickens to approaches that use replication-competent retroviruses. The need to produce transgenic chickens in the absence of replicating virus prompted development of a new method of gene transfer into the chicken. Microinjection of the replication-defective reticuloendotheliosis virus (REV) vector ME111 beneath unincubated chicken embryo blastoderms results in infection of germline stem cells. This vector contains genetic information exogenous to the chicken genome, including both the herpes simplex virus type 1 thymidine kinase gene and the Tn5 neomycin phosphotransferase gene. About 8 percent of male birds hatched from injected embryos contained vector DNA in their semen. All four positive males tested passed vector sequences onto their progeny. Analysis of G1 offspring showed that gonads of G0 male birds were mosaic with respect to insertion of vector provirus. Thus, primordial germ cells present in the unincubated chicken embryo blastoderm are susceptible to infection by defective REV vectors.

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Additional Information:© 1989 American Association for the Advancement of Science. 26 September 1988; Accepted 2 December 1988. We thank H. Temin for providing the C3 helper cells, M. Emerman for advice on the ME1 1 vector, N. Davidson for continuing interest in this work, A. Berk for critical reading of the manuscript, and J. Bennett and J. Heuston for manuscript preparation.
Issue or Number:4890
Record Number:CaltechAUTHORS:20150116-145551262
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Official Citation:Bosselman, R., Hsu, R., Boggs, T., Hu, S., Bruszewski, J., Ou, S., . . . et, a. (1989). Germline transmission of exogenous genes in the chicken. Science, 243(4890), 533-535. doi: 10.1126/science.2536194
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:53840
Deposited On:17 Jan 2015 03:16
Last Modified:10 Nov 2021 20:07

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